Gold Nanoparticle Flow Sensors Designed for Dynamic X-ray Imaging in Biofluids
Gold Nanoparticle Flow Sensors Designed for Dynamic X-ray Imaging in Biofluids
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DOI：
10.1021/nn1003293
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发表时间：
2010-07-01
期刊：
影响因子：
17.1
通讯作者：
Lee, Sang Joon
中科院分区：
文献类型：
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作者：
Ahn, Sungsook;Jung, Sung Yong;Lee, Sang Joon
基于X射线的成像是最强大和最方便的方法之一，因为它具有适用能量的多功能性和使用中的高性能。与传统的核医学成像不同，X射线成像需要造影剂，特别是为了有效靶向和分子特异性功能。在这里，与许多报道的造影剂在靶器官中的静态积累相反，首次通过粒子跟踪X射线成像成功地实现了活生物体中的动态可视化。在水稻木质部导管穿孔端壁的流动现象进行监测的金纳米粒子（AuNP）（类似于20 nm的直径）作为一个流动跟踪传感器工作在不透明的生物流体。金纳米颗粒表面改性，以控制流体动力学性质，如流体动力学尺寸（D-H），ζ电位，和表面等离子体性质在水性条件下。透射电子显微镜（TEM）、扫描电子显微镜（SEM）、X射线纳米显微镜（XN）和X射线显微镜（XM）用于将颗粒间相互作用与X射线吸收能力相关联。簇的形成和X射线对比度的能力的金纳米粒子成功地调制通过控制粒子间的相互作用作为流跟踪传感器进行评估。
X-ray-based imaging is one of the most powerful and convenient methods in terms of versatility in applicable energy and high performance in use. Different from conventional nuclear medicine imaging, contrast agents are required in X-ray imaging especially for effectively targeted and molecularly specific functions. Here, in contrast to much reported static accumulation of the contrast agents in targeted organs, dynamic visualization in a living organism is successfully accomplished by the particle-traced X-ray imaging for the first time. Flow phenomena across perforated end walls of xylem vessels in rice are monitored by a gold nanoparticle (AuNP) (similar to 20 nm in diameter) as a flow tracing sensor working in nontransparent biofluids. AuNPs are surface-modified to control the hydrodynamic properties such as hydrodynamic size (D-H), zeta-potential, and surface plasmonic properties in aqueous conditions. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray nanoscopy (XN), and X-ray microscopy (XM) are used to correlate the interparticle interactions with X-ray absorption ability. Cluster formation and X-ray contrast ability of the AuNPs are successfully modulated by controlling the interparticle interactions evaluated as flow-tracing sensors.