Immunolocalization of regenerating cells after submassive liver necrosis using PCNA staining
Immunolocalization of regenerating cells after submassive liver necrosis using PCNA staining
复制标题
使用 PCNA 染色对大面积肝坏死后再生细胞进行免疫定位
作者：
G. Koukoulis;A. Rayner;K. Tan;Roger Williams;B. Portmann
关于人类急性肝功能衰竭（ALF）中肝细胞增殖状态及其与各种形态学表现的关系的数据很少。（克隆PC-10）对增殖细胞核抗原（PCNA）检测3个正常肝脏石蜡切片中的周期细胞，对乙酰氨基酚过量（POD）导致的亚大块肝坏死（SHN）的14份尸检穿刺标本，以及在假定病毒病因的急性或亚急性肝功能衰竭患者移植时获得的10份肝切除标本。在正常肝脏中，仅偶见血窦衬里细胞染色，而在POD或假定病毒性肝炎后的SHN中，不同形态的肝细胞显示出显著的免疫反应性。POD后，5-6天内采集的样本的免疫反应性高于9-11天采集的样本，这一模式让人想起了先前在人类部分肝切除术后记录的再生率下降。免疫标记的肝细胞聚集在多腺泡“结节”中，具有塌陷和非塌陷实质的地图样分布。小管表现出相对较少的染色，但广泛的标记，特别是在完全肝细胞脱落的地区。在这些区域中，具有强PCNA阳性卵圆形核的小细长细胞，形成孔周发芽索或并入小导管的衬里中，最引人注目的是它们与动物研究中描述的“卵圆细胞”非常相似。
Little data exist on the proliferative state of liver cells and its relationship with various morphological findings in acute liver failure (ALF) in man. In this study we used the monoclonal antibody NCL‐PCNA (clone PC‐10) against the proliferating cell nuclear antigen (PCNA) to detect cycling cells in paraffin sections of 3 normal livers, 14 post‐mortem needle‐specimens of submassive hepatic necrosis (SHN) due to paracetamol overdosage (POD), and 10 hepatectomy specimens obtained at transplantation in patients with acute or subacute liver failure of presumed viral aetiology. In normal livers, only occasional sinusoid‐lining cells were stained, whereas in SHN following POD or presumed viral hepatitis, hepatocytes of variable morphology showed significant immunoreactivity. Following POD, immuno‐reactivity was higher in samples taken within 5–6 days than in those obtained at 9–11 days, a pattern reminiscent of the decrease in the rate of regeneration, previously documented after partial hepatectomy in humans. Immunolabelled hepatocytes were aggregated in multiacinar ‘nodules’ in cases with a map‐like distribution of collapsed and non‐collapsed parenchyma. Ductules demonstrated comparatively less staining, but extensive labelling was exceptionally found in areas of complete hepatocellular dropout. In these areas, small elongated cells with strongly PCNA‐positive ovoid nuclei, forming periporal sprouting cords or incorporated into the lining of ductules, were most remarkable in that they closely resembled ‘oval cells’ described in animal studies.
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DOI：
10.1073/pnas.83.13.4739
发表时间：
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影响因子：
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DOI：
--
发表时间：
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期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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DOI：
--
发表时间：
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影响因子：
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通讯作者：
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