Characterization and Biological Activities of Seed Oil Extracted from Berberis dasystachya Maxim. by the Supercritical Carbon Dioxide Extraction Method
Characterization and Biological Activities of Seed Oil Extracted from Berberis dasystachya Maxim. by the Supercritical Carbon Dioxide Extraction Method
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DOI：
10.3390/molecules25081836
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发表时间：
2020-04
期刊：
影响因子：
4.6
通讯作者：
Lijuan Han;Qingqing Han;Yongjing Yang;Honglun Wang;Shulin Wang;Gang Li
中科院分区：
文献类型：
--
作者：
Lijuan Han;Qingqing Han;Yongjing Yang;Honglun Wang;Shulin Wang;Gang Li
传统的藏药和食用果实--黄连的结构和药理活性尚未见报道。采用中心组合设计法和响应面分析法相结合的方法，对超临界二氧化碳萃取法(SC-CO2)提取罗汉果油(BDSO)的工艺条件进行了优化，并与石油醚萃取法(PEE)进行了比较。分析了BDSO的化学性质，并用DPPH、ABTS、还原功率法、四甲基偶氮唑盐比色法研究了BDSO的抗氧化活性和体外细胞存活率。结果表明，在压力为25.00 MPa、温度为59.03℃、CO2流量为2.25SL/min的最佳提取条件下，茶多酚的得率最高，为12.54±0.56g/100g。气相色谱分析结果表明，超临界CO2法提取的BDSO中不饱和脂肪酸含量(85.62%)和多不饱和脂肪酸含量(57.90%)均高于PEE法提取的BDSO。气相色谱与离子迁移率谱(GC-IMS)联用分析结果表明，BDSO中的主要挥发性化合物为醛和酯。BDSO还表现出剂量依赖性的抗氧化能力。此外，与BDSO孵育的正常细胞和癌细胞的存活率都在85%以上，表明BDSO没有细胞毒性。基于这些结果，SC-CO2法提取的BDSO可能被用于其他应用，例如那些涉及使用B.dasystachya浆果的应用。
Characterization of the structure and pharmacological activity of Berberis dasystachya Maxim., a traditional Tibetan medicinal and edible fruit, has not yet been reported. In this study, central composite design (CCD) combined with response surface methodology (RSM) was applied to optimize the extraction conditions of B. dasystachya oil (BDSO) using the supercritical carbon dioxide (SC-CO2) extraction method, and the results were compared with those obtained by the petroleum ether extraction (PEE) method. The chemical characteristics of BDSO were analyzed, and its antioxidant activity and in vitro cellular viability were studied by DPPH, ABTS, reducing power assay, and MTT assay. The results showed that the maximum yield of 12.54 ± 0.56 g/100 g was obtained at the optimal extraction conditions, which were: pressure, 25.00 MPa; temperature 59.03 °C; and CO2 flow rate, 2.25 SL/min. The Gas chromatography (GC) analysis results showed that BDSO extracted by the SC-CO2 method had higher contents of unsaturated fatty acids (85.62%) and polyunsaturated fatty acids (57.90%) than that extracted by the PEE method. The gas chromatography used in conjunction with ion mobility spectrometry (GC–IMS) results showed that the main volatile compounds in BDSO were aldehydes and esters. BDSO also exhibited antioxidant ability in a dose-dependent manner. Moreover, normal and cancer cells incubated with BDSO had survival rates of more than 85%, which indicates that BDSO is not cytotoxic. Based on these results, the BDSO extracted by the SC-CO2 method could potentially be used in other applications, e.g., those that involve using berries of B. dasystachya.