Role of 5 Saponins in Secretion of Cytokines by PRRSV-induced Endothelial Cells
Role of 5 Saponins in Secretion of Cytokines by PRRSV-induced Endothelial Cells
复制标题
5 种皂苷在 PRRSV 诱导的内皮细胞分泌细胞因子中的作用。
DOI：
10.1055/s-0042-106577
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发表时间：
2016-07-01
期刊：
影响因子：
2.2
通讯作者：
He, K.
中科院分区：
文献类型：
--
作者：
Hu, Y.;Mao, A.;He, K.
The aim of the research presented here was to investigate the immunoloregulatory effects of 5 saponins: Saikosaponins A (SSA) and D (SSD) from Bupleurum chinese DC (Umbelliferae), Panax Notoginseng Saponin (PNS) and Notoginsenoside R1 from Panax notoginseng (Araliaceae) (SR1), and Anemoside B4 from Pulsatilla chinensis Regel (Ranunculaceae) (AB4). To achieve this, endothelial cells were challenged with 10(5) TCID50/mL PRRSV for 24 h then treated respectively with 5 saponins at 3 concentrations (1, 5 and 10 mu g/mL). The cells were incubated at 37 degrees C in a cell incubator for 24 h. The supernatants were collected and analyzed the levels of interleukin-4 (IL-4), IL-10, IL-2, and gamma-interferon (IFN-gamma.) by ELISA kits. The results revealed that PNS and SR1 inhibited the production of IL-4; PNS, SR1 and SSD inhibited the secretion of IL-10; SSA, SSD and AB4 up-regulated IL-2 expression; SSA and SSD increased the level of IFN-gamma. All these changes were significant. Taken together, the data suggested that these 5 saponins might effectively regulate immune responses via changes in the levels of these select cytokines.
