Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool.
Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool.
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DOI：
10.3201/eid2202.151796
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发表时间：
2016-02
影响因子：
11.8
通讯作者：
Feldmann H
中科院分区：
文献类型：
--
作者：
Hoenen T;Groseth A;Rosenke K;Fischer RJ;Hoenen A;Judson SD;Martellaro C;Falzarano D;Marzi A;Squires RB;Wollenberg KR;de Wit E;Prescott J;Safronetz D;van Doremalen N;Bushmaker T;Feldmann F;McNally K;Bolay FK;Fields B;Sealy T;Rayfield M;Nichol ST;Zoon KC;Massaquoi M;Munster VJ;Feldmann H
对疾病暴发期间获得的病原体样本进行RNA/DNA快速测序，可提供重要的科学和公共卫生信息。然而，在向实验室出口样本或在偏远疫情地区建立常规测序仪方面存在挑战。我们成功地使用了一种新的，口袋大小的纳米孔测序仪在现场诊断实验室在利比里亚在目前的埃博拉病毒爆发。
Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.