METHODS FOR NONLETHAL GILL BIOPSY AND MEASUREMENT OF NA+, K+ -ATPASE ACTIVITY
METHODS FOR NONLETHAL GILL BIOPSY AND MEASUREMENT OF NA+, K+ -ATPASE ACTIVITY
复制标题
DOI：
10.1139/f93-075
复制
发表时间：
1993-03-01
影响因子：
2.4
通讯作者：
MCCORMICK, SD
中科院分区：
文献类型：
--
作者：
MCCORMICK, SD
从麻醉的鱼身上取出一小部分鳃组织的鳃活检，被证明对随后的大西洋鲑鱼幼鱼(Salmo Salar)的存活、生长和耐盐性没有不利影响。本文提出了一种测定这少量鳃组织中Na~+，K~+-ATPase活性的方法。这些方法可用于非致命性监测鲑类的生理气味特征，并可能在许多鱼类的疾病、毒理学和生理生态学的研究中应用。
A gill biopsy, in which a small portion of gill tissue was removed from anesthetized fish, was shown to have no detrimental effect on subsequent survival, growth, and salinity tolerance of juvenile Atlantic salmon (Salmo salar). A method for measurement of Na+, K+-ATPase activity in this small amount of gill tissue is presented. These methods are useful for nonlethal monitoring of physiological smolt characteristics in salmonids and may have applications in the study of disease, toxicology, and physiological ecology of many fish species.