A Myocardial Patch Made of Collagen Membranes Loaded with Collagen-Binding Human Vascular Endothelial Growth Factor Accelerates Healing of the Injured Rabbit Heart
A Myocardial Patch Made of Collagen Membranes Loaded with Collagen-Binding Human Vascular Endothelial Growth Factor Accelerates Healing of the Injured Rabbit Heart
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DOI：
10.1089/ten.tea.2011.0105
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发表时间：
2011-11-01
影响因子：
4.1
通讯作者：
Dai, Jianwu
中科院分区：
文献类型：
--
作者：
Gao, Jian;Liu, Jianzhou;Dai, Jianwu
组织工程化心肌补片可用于修复心肌损伤。本研究的目的是评价一种用于心肌修复的胶原靶向递送系统。将胶原结合结构域(CBD)与人血管内皮细胞生长因子(VEGF)融合，以增强其与胶原的结合。在本研究中，胶原膜负载CBD-血管内皮生长因子、天然血管内皮生长因子或磷酸盐缓冲液作为心脏补片用于修复兔心肌梗死模型。与其他两组比较，CBD-VEGF/胶原蛋白复合组在4周后能有效地诱导更多的细胞进入胶原膜，12周后能促进更多的梗塞心肌血管形成。超声心动图和血流动力学研究均显示CBD-VEGF/胶原组的心功能有所改善。这些结果表明，CBD-VEGF胶原膜贴片植入梗死区心肌能有效改善左心功能，增加血管密度。
Tissue-engineered myocardial patches could be useful in the repair of myocardial injuries. The aim of the present study was to evaluate a collagen targeting delivery system for myocardial repair. A specific peptide collagen-binding domain (CBD) was fused to human vascular endothelial growth factor (VEGF) to enhance the binding of VEGF to collagen. In this study, collagen membranes loaded with CBD-VEGF, natural VEGF, or phosphate-buffered saline are used as cardiac patches to repair the infarcted myocardium in a rabbit model. CBD-VEGF/collagen group could effectively induce more cells to penetrate into the collagen membrane after 4 weeks and promote more vascularization in infarcted myocardium after 12 weeks compared with the other two control groups. Echocardiography and hemodynamic studies both show cardiac function improvement in the CBD-VEGF/collagen group. These results reveal that implantation of CBD-VEGF collagen membrane patch into the infarcted myocardium could effectively improve left ventricle cardiac function and increase the vascular density.