Proteomic Analysis of Protein Expression in the Induction of the Viable But Nonculturable State of Vibrio harveyi SF1
Proteomic Analysis of Protein Expression in the Induction of the Viable But Nonculturable State of Vibrio harveyi SF1
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DOI：
10.1007/s00284-013-0383-9
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发表时间：
2013-10-01
影响因子：
2.6
通讯作者：
Liu, Mengzhen
中科院分区：
文献类型：
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作者：
Jia, Juntao;Li, Zhengyi;Liu, Mengzhen
据报道，哈韦氏弧菌已进入可存活但不可培养（VBNC）状态。一种海洋哈维氏弧菌菌株，SF1在4℃的海水微观环境中培养60天后变得不可培养。我们研究了V. harveyi SF1在指数期的蛋白表达，并将其与VBNC状态进行了比较。细胞质蛋白通过二维聚丙烯酰胺凝胶电泳在pH 4-7线性梯度下分离。通过MALDI-TOF-TOF质谱法鉴定了其中16个在VBNC细胞中强烈下调或上调的蛋白。结果表明，这些差异表达蛋白主要集中在应激反应蛋白和碳水化合物代谢、转运和翻译等中、中间代谢的关键成分。本研究为了解VBNC状态的适应机制提供了线索。
Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 A degrees C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cytosolic proteins were resolved by two-dimensional polyacrylamide gel electrophoresis using pH 4-7 linear gradients. Among these proteins, sixteen proteins which were strongly downregulated or upregulated in the VBNC cells were identified by MALDI-TOF-TOF mass spectrometry. The results indicated that the differentially expressed proteins were mainly focused on stress response proteins and key components of central and intermediary metabolism, like carbohydrate metabolism, transport, and translation. This study provided clues for understanding the mechanism of adaptation to the VBNC state.