Kinetic study of DNA hybridization on DNA-modified gold nanoparticles with engineered nano-interfaces

Kinetic study of DNA hybridization on DNA-modified gold nanoparticles with engineered nano-interfaces
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DOI:
10.1039/c5ra13116b
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发表时间:
2015-09
期刊:
影响因子:
3.9
通讯作者:
Akari Takashima;Motoi Oishi
Akari Takashima;Motoi Oishi
中科院分区:
化学3区
文献类型:
--
作者:
Akari Takashima;Motoi Oishi

文献摘要

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使用DNA修饰的金纳米颗粒(GNP)的比色DNA传感系统依赖于GNP上的DNA杂交事件。我们报告了一个系统的研究与不同的表面修饰的GNP使用探针DNA和不同分子量的PEG的DNA杂交动力学。DNA杂交动力学不仅对探针DNA密度而且对PEG的分子量极其敏感。
Colorimetric DNA sensing systems using DNA-modified gold nanoparticles (GNPs) rely on DNA hybridization events on the GNP. We report a systematic study of DNA hybridization kinetics on GNPs with different surface modifications using probe DNA and different molecular weight PEGs. The DNA hybridization kinetics are extremely sensitive not only to probe DNA density but also to the molecular weight of PEG.