Drosophila Heterochromatin Stabilization Requires the Zinc-Finger Protein Small Ovary
Drosophila Heterochromatin Stabilization Requires the Zinc-Finger Protein Small Ovary
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DOI：
10.1534/genetics.119.302590
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发表时间：
2019-11-01
期刊：
影响因子：
3.3
通讯作者：
Lerit, Dorothy A.
中科院分区：
文献类型：
--
作者：
Benner, Leif;Castro, Elias A.;Lerit, Dorothy A.
异染色质介导的阻遏对于控制转座子的表达和协调细胞类型特异性基因调控是必不可少的。小卵巢（sov）基因座被确定在筛选雌性不育突变的果蝇，突变体显示戏剧性的卵巢形态发生缺陷。我们表明，空sov表型是致命的，并映射到未表征的基因，它编码一个核锌指蛋白，共定位与必要的异染色质蛋白1（HP1a）的基因座。我们证明了Sov的功能，抑制不适当的基因表达在卵巢，沉默转座子，并抑制位置效应杂色在眼睛，这表明在异染色质稳定的核心作用。
Heterochromatin-mediated repression is essential for controlling the expression of transposons and for coordinated cell type-specific gene regulation. The small ovary (sov) locus was identified in a screen for female-sterile mutations in Drosophila melanogaster, and mutants show dramatic ovarian morphogenesis defects. We show that the null sov phenotype is lethal and map the locus to the uncharacterized gene , which encodes a nuclear zinc-finger protein that colocalizes with the essential Heterochromatin Protein 1 (HP1a). We demonstrate Sov functions to repress inappropriate gene expression in the ovary, silence transposons, and suppress position-effect variegation in the eye, suggesting a central role in heterochromatin stabilization.