Directional fluid induced self-assembly of oriented bacterial cellulose nanofibers for potential biomimetic tissue engineering scaffolds
Directional fluid induced self-assembly of oriented bacterial cellulose nanofibers for potential biomimetic tissue engineering scaffolds
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DOI：
10.1016/j.matchemphys.2014.10.037
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发表时间：
2015-01
影响因子：
4.6
通讯作者：
Y. Wan;Da Hu;Guang-yao Xiong;Deying Li;R. Guo;Honglin Luo
中科院分区：
文献类型：
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作者：
Y. Wan;Da Hu;Guang-yao Xiong;Deying Li;R. Guo;Honglin Luo
在本工作中，利用一种能为细菌纤维素(BC)培养产生定向流体环境的新型发酵罐来生产取向的BC(OBC)纳米纤维。用扫描电子显微镜、X射线衍射仪、傅立叶变换红外光谱和力学性能测试等手段对所制备的炭黑进行了表征。研究发现，在定向流体的作用下，OBC纳米纤维能够很好地取向，可以制备出大尺寸的OBC支架。XRD和FT-IR分析表明，定向流体并不影响化学结构，但与静态培养条件下制备的BC相比，OBC的结晶度有所降低。拉伸测试表明，由于取向的纤维结构，力学性能在两个垂直方向上具有各向异性。OBC有望成为引导组织定向生长的仿生支架材料。
In this work, a novel fermentor which can generate directional fluid environment for the cultivation of bacterial cellulose (BC) was used to produce oriented BC (OBC) nanofibers. The as-prepared OBC was characterized by SEM, XRD, FT-IR, and mechanical testing. It is found that OBC nanofibers are well aligned under the action of directional fluid and large-sized OBC scaffolds can be produced. XRD and FT-IR analyses indicate that directional fluid does not affect the chemical structure while the crystallinity of OBC decreases as compared to BC produced under static cultivation conditions. Tensile testing reveals the anisotropy of the mechanical properties in two orthogonal directions due to the oriented fiber structure. It is expected that OBC may be applied as biomimetic scaffolds for guided directional tissue growth.