CELL LINEAGE ANALYSIS BY INTRACELLULAR INJECTION OF FLUORESCENT TRACERS
CELL LINEAGE ANALYSIS BY INTRACELLULAR INJECTION OF FLUORESCENT TRACERS
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DOI：
10.1126/science.6159680
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发表时间：
1980-01-01
期刊：
影响因子：
56.9
通讯作者：
YOUNG, JD
中科院分区：
文献类型：
--
作者：
WEISBLAT, DA;ZACKSON, SL;YOUNG, JD
水蛭[Helobdella triserialis]发育过程中的细胞谱系通过将荧光肽罗丹明-D-肽注射到已鉴定的胚胎细胞中来揭示。该肽与核染色一起显示干细胞及其后代的定型裂解模式。罗丹明-D-肽和链霉蛋白酶的联合注射证明了在链霉蛋白酶注射的成端细胞中干细胞生产的停滞。
Cell lineages during development of the leech [Helobdella triserialis] are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. The peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.