Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes
Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes
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DOI：
10.1099/mic.0.28030-0
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发表时间：
2005-08-01
期刊：
影响因子：
2.8
通讯作者：
Golden, SS
中科院分区：
文献类型：
--
作者：
Ditty, JL;Canales, SR;Golden, SS
KaIa、Kaib和KaiC基因编码长聚球藻PCC7942中蓝藻生物钟的核心成分。KaIA和KaiBC操纵子的节律表达模式通常同步达到峰值。在一些突变体中，这些转录单位之间的峰值的相对时间(相位关系)被改变，但昼夜节律保持强劲。本研究探讨了KAI基因转录时序的重要性。从一个异源启动子表达KaIA或KaiBC，其峰值出现在与正常位点异相12小时，因此与另一个KAI基因座异相12小时，生物发光记者测量，不影响一个周期(周期)或昼夜节律阶段所需的时间。此外，这些数据证实了KAI基因启动子中的特定顺式元件不是维持时钟功能所必需的。
The kaiA, kaiB and kaiC genes encode the core components of the cyanobacterial circadian clock in Synechococcus elongatus PCC 7942. Rhythmic expression patterns of kaiA and of the kaiBC operon normally peak in synchrony. In some mutants the relative timing of peaks (phase relationship) between these transcription units is altered, but circadian rhythms persist robustly. In this study, the importance of the transcriptional timing of kai genes was examined. Expressing either kaiA or kaiBC from a heterologous promoter whose peak expression occurs 12 In out of phase from the norm, and thus 12 h out of phase from the other kai locus, did not affect the time required for one cycle (period) or phase of the circadian rhythm, as measured by bioluminescence reporters. Furthermore, the data confirm that specific cis elements within the promoters of the kai genes are not necessary to sustain clock function.