Switchable Fluorophores for Single-Molecule Localization Microscopy.
Switchable Fluorophores for Single-Molecule Localization Microscopy.
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用于单分子定位显微镜的可切换荧光团。
DOI:
10.1021/acs.chemrev.7b00767
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发表时间:
2018-09-26
期刊:
影响因子:
62.1
通讯作者:
Vaughan JC
中科院分区:
文献类型:
--
作者:
Li H;Vaughan JC
The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.
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