Switchable Fluorophores for Single-Molecule Localization Microscopy.

Switchable Fluorophores for Single-Molecule Localization Microscopy.
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用于单分子定位显微镜的可切换荧光团。

DOI:
10.1021/acs.chemrev.7b00767
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发表时间:
2018-09-26
期刊:
影响因子:
62.1
通讯作者:
Vaughan JC
Vaughan JC
中科院分区:
化学1区
文献类型:
--
作者:
Li H;Vaughan JC

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在过去的十年里,超分辨率荧光显微镜方法在生物学和其他领域的应用出现了爆炸式增长。单分子定位显微镜(SMLM)是这些方法中最广泛的一种,其成功在很大程度上归功于通过各种化学、光化学或结合-解结合机制控制荧光团的开-关状态的能力。我们在这里提供了一个全面的概述可切换的荧光团在SMLM,包括所有主要类别的SMLM荧光团的详细审查,我们还解决了标记标本的策略,考虑多通道和活细胞成像,潜在的陷阱,以及未来的发展领域。
The past decade has witnessed an explosion in the use of super-resolution fluorescence microscopy methods in biology and other fields. Single-molecule localization microscopy (SMLM) is one of the most widespread of these methods and owes its success in large part to the ability to control the on-off state of fluorophores through various chemical, photochemical, or binding-unbinding mechanisms. We provide here a comprehensive overview of switchable fluorophores in SMLM including a detailed review of all major classes of SMLM fluorophores, and we also address strategies for labeling specimens, considerations for multichannel and live-cell imaging, potential pitfalls, and areas for future development.
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