FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells
FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells
复制标题
DOI：
10.1080/08977190701345200
复制
发表时间：
2007-01-01
期刊：
影响因子：
1.8
通讯作者：
Bayes-Genis, Antoni
中科院分区：
文献类型：
--
作者：
Farre, Jordi;Roura, Santiago;Bayes-Genis, Antoni
人骨髓间充质干细胞（MSCs）在体外生长有限。成纤维细胞生长因子（FGF）可引起细胞增殖、分化和迁移等多种生物学反应。成纤维细胞生长因子-4（FGF-4）是具有最高促细胞有丝分裂活性的成纤维细胞生长因子之一，我们研究了FGF-4对骨髓间充质干细胞生长和多能性的影响。与对照相比，FGF-4显著降低了MSC的复制时间（Td）（分别为2.2 +/-0.2天对4.1 +/-0.2天; p=0.03），而BMP-2和SCF-1没有发挥显著的生长作用。MSC表面标志物的表达、成脂和成骨谱系的分化以及心肌细胞基因的基线表达不受FGF-4的影响。总之，外源性FGF-4增加MSC增殖的速率，并且对MSC多能性没有显著影响。
Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity.We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 +/- 0.2 vs. 4.1 +/- 0.2 days, respectively; p=0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4.In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.