Reproducibility in quality control of protein (Western) immunoblot assay for antibodies to human immunodeficiency virus.
Reproducibility in quality control of protein (Western) immunoblot assay for antibodies to human immunodeficiency virus.
复制标题
人类免疫缺陷病毒抗体的蛋白质（西方）免疫印迹测定质量控制的重现性。
DOI：
10.1093/ajcp/91.1.75
复制
发表时间：
1989
影响因子：
3.5
通讯作者：
Mosley,JW
中科院分区：
文献类型：
--
作者：
Edwards,VM;Mosley,JW
人类免疫缺陷病毒抗体的蛋白质（Western）免疫印迹试验（IB），像其他实验室程序一样，有时会给出不同的结果。在输血安全研究中，每组患者标本常规提交了四种质量控制（QC）样本的不可区分的等份。两种已知阳性（QC）标准的179次测定中有1.7%的IB结果为假阴性，两种已知阴性（QC）标准的101次测定中有2.0%的IB结果为假阳性。此外，一次向三个广泛使用的实验室送去了一个由24个样品组成的测试小组。一个实验室报告了假阳性结果，另一个实验室报告了假阴性结果。虽然总体上是可靠的，但IB结果偶尔也会出错。抗原-抗体条带的相对频率在技术上的可变性比人们认识到的要大得多。这些实验室间和实验室内的比较表明，质量控制检查对所有实验室都是必不可少的，如果对特定患者的适用性有疑问，则应检测多个标本。特定波段是足够不恒定的同一标本，使出现或消失的连续标本的预后不可靠。
The protein (Western) immunoblot assay (IB) for antibodies to human immunodeficiency virus, like other laboratory procedures, sometimes gives variable results. In the Transfusion Safety Study, indistinguishable aliquots of four quality control (QC) samples have been routinely submitted for IB with each group of patient specimens. A false negative IB result was obtained for 1.7% of 179 assays of two known positive (QC) standards and a false positive result for 2.0% of 101 assays of two known negative (QC) standards. In addition, a test panel of 24 samples was sent on a single occasion to three widely used laboratories. A false positive result was reported by one laboratory and a false negative by a second. Although generally reliable, IB results may occasionally be in error. There is much more technical variability in the relative frequencies of antigen–antibody bands than has been recognized. These interlaboratory and intralaboratory comparisons show quality control checks are essential for all laboratories, and more than one specimen should be tested if its applicability to a specific patient is questionable. Specific bands are sufficiently inconstant for the same specimen to make appearance or disappearance on successive specimens prognostically unreliable.