Relation between endothelial cell apoptosis and blood flow direction in human atherosclerotic plaques
Relation between endothelial cell apoptosis and blood flow direction in human atherosclerotic plaques
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DOI：
10.1161/01.cir.101.21.2450
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发表时间：
2000-05-30
期刊：
影响因子：
37.8
通讯作者：
Tedgui, A
中科院分区：
文献类型：
--
作者：
Tricot, O;Mallat, Z;Tedgui, A
背景-血流特征影响内皮细胞凋亡，然而，很少有人知道内皮细胞凋亡的发生在人类动脉粥样硬化及其与血流的关系。方法和结果-共42人颈动脉粥样硬化斑块动脉内膜切除术检索，他们在纵向轴向进行了检查。当上游和下游部分清晰可见，不存在闭塞，并且沿斑块沿着均存在管腔内皮的免疫染色时，将斑块纳入本研究。使用这些标准，处理13个斑块，用于进一步免疫组织化学研究（使用抗CD 31、抗Ki-67和抗剪接因子抗体）和原位检测细胞凋亡（末端dUTP缺口末端标记和连接酶测定）。8个斑块在管腔表面显示大于或等于1个凋亡内皮细胞。对这些斑块中内皮细胞凋亡的定量分析显示，与斑块的上游部分相比，在低血流和低切应力占优势的斑块下游部分系统性优先发生凋亡（分别为18.8 ± 3.3%和2.7 ± 1.2%，P
Background-Blood flow characteristics influence endothelial cell apoptosis, However, little is known about the occurrence of endothelial cell apoptosis in human atherosclerosis and its relation to blood flow.Methods and Results-A total of 42 human carotid atherosclerotic plaques were retrieved by endarterectomy; they were examined in the longitudinal axial direction. Plaques were included in this study when upstream and downstream parts were clearly visible, occlusion was absent, and immunostaining for luminal endothelium was present all along the plaque. Using these criteria, 13 plaques were processed For Further immunohistochemical studies (using anti-CD31, anti-Ki-67, and anti-splicing factor antibodies) and in situ detection of apoptosis (terminal dUTP nick end-labeling and ligase assay). Eight plaques showed greater than or equal to 1 apoptotic endothelial cell at the luminal surface. Quantitative analysis of endothelial cell apoptosis in these plaques showed a systematic preferential occurrence of apoptosis in the downstream parts of plaques, where low flow and low shear stress prevail, in comparison with the upstream parts (18.8+/-3.3% versus 2.7+/-1.2%, respectively, P