A proteomic analysis of Streptomyces coelicolor programmed cell death
A proteomic analysis of Streptomyces coelicolor programmed cell death
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DOI：
10.1002/pmic.200600147
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发表时间：
2006-11-01
期刊：
影响因子：
3.4
通讯作者：
Sanchez, Jesus
中科院分区：
文献类型：
--
作者：
Manteca, Angel;Maeder, Ulrike;Sanchez, Jesus
细胞程序性死亡（PCD）是真核生物和细菌在生物和非生物胁迫下发生的一种主动的细胞自杀。与真核细胞凋亡相反，对调控细菌PCD的分子机制知之甚少。在先前的工作中，我们描述了链霉菌中PCD现象的存在（曼特卡等人，微生物研究2006，157，143152）。在本研究中，我们进行了蛋白质组学分析的PCD天蓝色链霉菌，为此，我们开发了一个系统，以获得死细胞和活细胞富集样品。这种丝状细菌中的PCD伴随着参与细胞大分子、调节蛋白和应激诱导蛋白降解的酶的出现。我们认为，这些蛋白质中的一些在PCD途径中具有特定的功能，并已提出了所鉴定的蛋白质的推定作用。几种抗氧化蛋白的增加表明氧化应激是细胞死亡的原因或结果。
Programmed cell death (PCD) is an active cellular suicide that occurs in eukaryotes and bacteria in response to both abiotic and biotic stresses. In contrast to eukaryotic apoptosis, little is known about the molecular machinery that regulates bacterial PCD. In a previous work, we described the existence of PCD phenomena in Streptomyces (Manteca et al., Res. Microbiol. 2006, 157,143152). In the present study, we performed a proteomic analysis of PCD in Streptomyces coelicolor, for which we developed a system to obtain dead and live cell-enriched samples. PCD in this filamentous bacterium is accompanied by the appearance of enzymes involved in the degradation of cellular macromolecules, regulatory proteins, and stress-induced proteins. We argue that some of these proteins have specific functions in the PCD pathway and putative roles for the identified proteins have been proposed. The increased amounts of several antioxidant proteins suggest oxidative stress as either the cause or consequence of the cell death.