Increasing the chemical potential of the germ-line antibody repertoire.
Increasing the chemical potential of the germ-line antibody repertoire.
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增加种系抗体库的化学潜力。
DOI：
10.1073/pnas.90.9.4008
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发表时间：
1993
影响因子：
11.1
通讯作者：
Lerner,R
中科院分区：
文献类型：
--
作者：
Sarvetnick,N;Gurushanthaiah,D;Han,N;Prudent,J;Schultz,P;Lerner,R
为了增加免疫库的化学潜力，已将金属离子结合轻链引入鼠基因组中。随后用荧光素缀合物免疫含有转基因的小鼠。在抗荧光素记忆B细胞隔室中以高频率发现转基因轻链。这种通用方法应适用于其他辅因子和小分子，并应导致产生具有独特催化活性的抗体。
To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.