Active and repressive chromatin are interspersed without spreading in an imprinted gene cluster in the mammalian genome.
Active and repressive chromatin are interspersed without spreading in an imprinted gene cluster in the mammalian genome.
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DOI：
10.1016/j.molcel.2007.06.024
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发表时间：
2007-08-03
期刊：
影响因子：
16
通讯作者：
Barlow DP
中科院分区：
文献类型：
--
作者：
Regha K;Sloane MA;Huang R;Pauler FM;Warczok KE;Melikant B;Radolf M;Martens JH;Schotta G;Jenuwein T;Barlow DP
Igf2r印迹簇是一种表观遗传沉默模型，其中ncRNA的表达使多个基因顺式沉默。在这里，我们绘制了一个250 kb的区域在小鼠胚胎成纤维细胞显示，组蛋白修饰与表达和沉默的基因是相互排斥的，并定位到离散的区域。表达的基因在启动子区由H3K4me3 + H3K4me2 + H3K9Ac修饰，并且在推定的调控元件上由H3K4me2 + H3K9Ac修饰。沉默基因表现出两种类型的不重叠的轮廓。一种类型分布在组织特异性沉默基因的大结构域上，仅包含H3K27me3。第二种类型在沉默印迹基因启动子和非表达假基因上形成局部灶，包含H3K9me3 + H4K20me3 ± HP 1。因此，哺乳动物的染色体臂含有活性染色质，其中散布着抑制性染色质，类似于以前被认为是着丝粒、端粒和非活性X染色体特征的异染色质类型。
The Igf2r imprinted cluster is an epigenetic silencing model in which expression of a ncRNA silences multiple genes in cis. Here, we map a 250 kb region in mouse embryonic fibroblast cells to show that histone modifications associated with expressed and silent genes are mutually exclusive and localized to discrete regions. Expressed genes were modified at promoter regions by H3K4me3 + H3K4me2 + H3K9Ac and on putative regulatory elements flanking active promoters by H3K4me2 + H3K9Ac. Silent genes showed two types of nonoverlapping profile. One type spread over large domains of tissue-specific silent genes and contained H3K27me3 alone. A second type formed localized foci on silent imprinted gene promoters and a nonexpressed pseudogene and contained H3K9me3 + H4K20me3 ± HP1. Thus, mammalian chromosome arms contain active chromatin interspersed with repressive chromatin resembling the type of heterochromatin previously considered a feature of centromeres, telomeres, and the inactive X chromosome.
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影响因子：
11.4
作者：
Sleutels, F;Tjon, G;Barlow, DP
通讯作者：
Barlow, DP
影响因子：
10.5
作者：
Mancini-DiNardo, Debora;Steele, Scott J. S.;Tilghman, Shirley M.
通讯作者：
Tilghman, Shirley M.
影响因子：
50.3
作者：
Liu, ZJ;Herlyn, M
通讯作者：
Herlyn, M
DOI：
10.1073/pnas.0408021101
发表时间：
2004-12-14
影响因子：
11.1
作者：
Chadwick, BP;Willard, HF
通讯作者：
Willard, HF
影响因子：
8
作者：
Mills, JJ;Falls, JG;Jirtle, RL
通讯作者：
Jirtle, RL