Development of 3D in vitro platform technology to engineer mesenchymal stem cells.
Development of 3D in vitro platform technology to engineer mesenchymal stem cells.
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DOI：
10.2147/ijn.s30434
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发表时间：
2012
影响因子：
8
通讯作者：
Domb AJ
中科院分区：
文献类型：
--
作者：
Hosseinkhani H;Hong PD;Yu DS;Chen YR;Ickowicz D;Farber IY;Domb AJ
本研究旨在建立一种三维体外培养系统，用于基因工程间充质干细胞（MSC）表达骨形态发生蛋白-2。我们借用纺纱行业的纳米制造技术，如静电纺丝，大量生产各种形状和尺寸的相同构建块，以制造由聚乙醇酸和胶原蛋白复合材料组成的静电纺丝纳米纤维片。将编码骨形态发生蛋白-2的质粒DNA水溶液与相同体积的阳离子多糖右旋精胺简单混合，形成了均匀的阳离子可生物降解的天然聚合物纳米颗粒。在将纳米颗粒掺入纳米纤维片中之前，将大鼠骨髓间充质干细胞培养在由聚乙醇酸和胶原蛋白复合材料组成的静电纺丝纳米纤维片上。与裸质粒DNA纳米纤维片培养的MSC相比，2天后在纳米纤维片上培养的MSC中检测到骨形态发生蛋白2。我们认为，将纳米颗粒掺入纳米纤维片是一种非常有前途的MSC基因工程策略，并可进一步应用于再生医学治疗。
This study aims to develop a three-dimensional in vitro culture system to genetically engineer mesenchymal stem cells (MSC) to express bone morphogenic protein-2. We employed nanofabrication technologies borrowed from the spinning industry, such as electrospinning, to mass-produce identical building blocks in a variety of shapes and sizes to fabricate electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen. Homogenous nanoparticles of cationic biodegradable natural polymer were formed by simple mixing of an aqueous solution of plasmid DNA encoded bone morphogenic protein-2 with the same volume of cationic polysaccharide, dextran-spermine. Rat bone marrow MSC were cultured on electrospun nanofiber sheets comprised of composites of poly (glycolic acid) and collagen prior to the incorporation of the nanoparticles into the nanofiber sheets. Bone morphogenic protein-2 was significantly detected in MSC cultured on nanofiber sheets incorporated with nanoparticles after 2 days compared with MSC cultured on nanofiber sheets incorporated with naked plasmid DNA. We conclude that the incorporation of nanoparticles into nanofiber sheets is a very promising strategy to genetically engineer MSC and can be used for further applications in regenerative medicine therapy.