Boric acid inhibits LPS-induced TNF-α formation through a thiol-dependent mechanism in THP-1 cells
Boric acid inhibits LPS-induced TNF-α formation through a thiol-dependent mechanism in THP-1 cells
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DOI：
10.1016/j.jtemb.2008.03.005
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发表时间：
2008-01-01
影响因子：
3.5
通讯作者：
Zhong, Laifu
中科院分区：
文献类型：
--
作者：
Cao, Jun;Jiang, Liping;Zhong, Laifu
氧化应激在炎症性疾病中起着重要作用，抗氧化剂的应用可以减少氧化应激，抑制炎症过程。硼已被牵连到调节某些炎症介质和调节炎症过程。我们研究了三肽谷胱甘肽（GSH）在调节硼酸（BA）油脂多糖（LPS）诱导的THP-1中肿瘤坏死因子α（TNF-α）形成的作用中的作用，发现BA对TNF-α的产生和细胞内GSH单核细胞均无显著影响。有趣的是，内容。而它可以抑制LPS诱导的TNF-α的形成，并改善D，L-丁硫氨酸-S，R-亚砜亚胺（BSO）诱导的GSH消耗。与BSO孵育24小时诱导细胞内GSH减少和TNF-α全部增加。用N-乙酰-L-半胱氨酸（NAC）处理没有显著增加细胞内GSH含量，但显著减少TNF-α的分泌。BSO预处理24 h进一步增强LPS诱导的TNF-α分泌和mRNA表达。BA抑制LPS刺激的TNF-α的形成也被BSO耗尽GSH后观察到。这些结果表明，BA可能在LPS刺激的炎症中具有抗炎作用，并且BA对TNF-α分泌的影响可能通过巯基依赖性机制诱导。(C)2008年Elsevier GmbH。All rights reserved.
Oxidative stress plays all important role during Inflammatory diseases and antioxidant administration to diminish oxidative stress may arrest inflammatory processes. Boron has been implicated to modulate certain inflammatory mediators and regulate inflammatory processes. Here we investigated the role of the tripeptide glutathione (GSH) in modulating the effects of boric acid (BA) oil lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-alpha) formation in THP-1 we found that BA had no significant effects on both TNF-alpha production and intracellular GSH monocytes. interestingly, contents. whereas it could inhibit LPS-induced TNF-alpha formation and ameliorated the D,L-buthionine-S,R-sulfoximine (BSO)-induced GSH depletion. Twenty-four hour incubation with BSO induced a decrease of the intracellular GSH and all increase of TNF-alpha. Treatment with N-acctyl-L-cysteine (NAC) did not significantly increase intracellular content of GSH but significantly reduced the secretion of TNF-alpha. BSO-pretreatment for 24 h enhanced the LPS-induced secretion and mRNA expression of TNF-alpha further. BA inhibited LPS-stimulated TNF-alpha formation was also seen after GSH depletion by BSO. These results indicate that BA may have anti-inflammatory effect in the LPS-stimulated inflammation and the effect of BA on TNF-alpha secretion may be induced via a thiol-dependent mechanism. (C) 2008 Elsevier GmbH. All rights reserved.