Two distinct forms of surface antigen gene rearrangement in Trypanosoma brucei
Two distinct forms of surface antigen gene rearrangement in Trypanosoma brucei
复制标题
布氏锥虫表面抗原基因重排的两种不同形式
DOI：
10.1038/297514a0
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发表时间：
1982
期刊：
影响因子：
64.8
通讯作者：
Richard O. Williams
中科院分区：
文献类型：
--
作者：
P. Majiwa;John R. Young;P. T. Englund;S. Z. Shapiro;Richard O. Williams
非洲锥虫在其哺乳动物宿主中依次表达不同的表面抗原1。不同的可变表面糖蛋白（VSG）抗原的表达与基因组DNA 2 -5的重排有关。几个实验室已经报道，一些VSG基因的表达伴随着在基因组中的新位置出现基因的重复拷贝3，4。然而，在一系列顺序相关的布氏锥虫克隆从不同的股票，我们无法检测到重复的一个VSG基因（ILTAT 1.2，B）的表达克隆。在表达和非表达克隆中发现该基因的两个拷贝。在两个基因拷贝的3′端附近发生明显的插入和缺失，但与表达无关2，5，6。我们在这里报告的观察两种类型的重排不同的VSG基因在同一系列的锥虫克隆。因此，不同类型的重排不是不同锥虫原种的功能，而是原种内不同VSG基因的功能。
African trypanosomes express different surface antigens sequentially in their mammalian hosts1. Expression of different variable surface glycoprotein (VSG) antigens is associated with rearrangements of genomic DNA2–5. Several laboratories have reported that expression of some VSG genes is accompanied by the appearance of a duplicated copy of the genes at a new location in the genome3,4. However, in a series of sequentially related Trypanosoma brucei clones from a different stock we were unable to detect duplication of one VSG gene (ILTAT 1.2, B) in expressing clones. Two copies of this gene were found in expressing and non-expressing clones. Apparent insertions and deletions occurred near the 3′ ends of both gene copies, but could not be correlated with expression2,5,6. We report here the observation of both types of rearrangement for different VSG genes in the same series of trypanosome clones. Thus the different types of rearrangement are not a function of different trypanosome stocks, but of different VSG genes within a stock.