The identification of flavonoids and the expression of genes of anthocyanin biosynthesis in the chrysanthemum flowers
The identification of flavonoids and the expression of genes of anthocyanin biosynthesis in the chrysanthemum flowers
复制标题
菊花中黄酮类化合物的鉴定及花色苷生物合成基因的表达
DOI：
10.1007/s10535-012-0069-3
复制
发表时间：
2012-09-01
影响因子：
1.5
通讯作者：
Zhang, Z.
中科院分区：
文献类型：
--
作者：
Chen, S. -M.;Li, C. -H.;Zhang, Z.
为了提供有关菊花颜色的更多信息，对粉红色花（cv. H5）和两种白色花（cvs. Keikai 和 Jinba）菊花品种的舌状花中黄酮类成分和涉及花青素途径的 6 个结构基因的表达进行了检测。 HPLCDAD/ESI-MSn分析表明，花青素3-O-(6″-O-丙二酰基葡萄糖苷)和花青素3-O-(3″,6″-O-二丙二酰基葡萄糖苷)是H5中的两种主要黄酮类化合物，而白花品种中含有黄酮类化合物而不是花青素。 3个品种中检测到9种黄酮衍生物，每种黄酮的含量因品种而异，其中7种黄酮衍生物被鉴定为木犀草素7-O-阿拉伯糖基葡萄糖苷、芹菜素7-O-葡萄糖苷、木犀草素7-O-丙二酰葡萄糖苷、芹菜素7-O-丙二酰葡萄糖苷、金酒醇7-O-丙二酰葡萄糖苷、金合欢素 7-O-芸香苷和金合欢素 7-O-丙二酰葡萄糖苷。两个白花品种的总黄酮含量相似，比 H5 高约两倍。仅在 H5 中检测到编码二氢黄酮醇 4-还原酶和 3-O-葡萄糖基转移酶的基因高表达，而在 Keikai 或 Jinba 中未检测到。查耳酮合酶、查耳酮异构酶、黄烷酮 3-羟化酶和类黄酮 3'-羟化酶在所有花中均表达，表明白花品种中花青素的缺乏不可能是由于其表达受阻所致。
In order to provide additional information on the coloration of chrysanthemum flowers, the flavonoid composition and the expression of six structural genes involved in anthocyanin pathway in the ray florets of a pink flowering (cv. H5) and two white flowering (cvs. Keikai and Jinba)Chrysanthemum grandiflorumcultivars were examined. HPLCDAD/ESI-MSnanalysis showed that cyanidin 3-O-(6″-O-malonylglucoside) and cyanidin 3-O-(3″,6″-O-dimalonylglucoside) were the two major flavonoids presented in H5, while white flowering cultivars contained flavones instead of anthocyanins. Nine flavone derivatives were detected in the three cultivars, the amount of each flavone varied upon cultivars, and seven of these were identified as luteolin 7-O-arabinosylglucuronide, apigenin 7-O-glucoside, luteolin 7-O-malonylglucoside, apigenin 7-O-malonylglucoside, chrysoeriol 7-O-malonylglucoside, acacetin 7-O-rutinoside and acacetin 7-O-malonylglucoside. The two white flowering cultivars showed similar total flavonoid content, which was about two fold higher than that in H5. A high expression of the genes encoding dihydroflavonol 4-reductase and 3-O-glucosyltransferase was detected only in H5 but not in Keikai or Jinba. Chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, and flavonoid 3′-hydroxylase were expressed in all flowers, suggesting that the lack of anthocyanin in white flowering cultivars cannot be due to any blockage of their expression.