Concentration of Norovirus during Wastewater Treatment and Its Impact on Oyster Contamination
Concentration of Norovirus during Wastewater Treatment and Its Impact on Oyster Contamination
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DOI：
10.1128/aem.07569-11
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发表时间：
2012-05-01
影响因子：
4.4
通讯作者：
Dore, William
中科院分区：
文献类型：
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作者：
Flannery, John;Keaveney, Sinead;Dore, William
大肠杆菌，F-特异性RNA噬菌体（FRNA噬菌体），诺如病毒基因组I（诺如病毒GI）和诺如病毒基因组II（诺如病毒GII）在废水中的浓度进行了监测，每周超过1年的时间在废水处理厂（WWTP）提供二级废水处理。共49个样品的进水废水和废水已处理的一级和二级废水处理工艺（一级和二级处理的废水）进行了分析。使用实时逆转录定量PCR（RT-qPCR），在流出废水中检测到的平均NoV GI和NoV GII浓度分别为2.53和2.63 log（10）病毒基因组拷贝100 ml（-1）。在冬季期间（1月至3月）（n = 12）的平均NoV浓度在废水中分别为0.82（NoV GI）和1.41（NoV GII）的对数单位大于平均浓度为其余的一年（n = 37）。治疗期间NoV GI和GII的平均降低分别为0.80和0.92 log单位，由于季节，未检测到NoV降低程度的显著差异。E.大肠杆菌或FRNA噬菌体的废水进水中，并显示平均减少1.49和2.13对数单位分别。在污水处理厂排放口附近的牡蛎样本中检测到的NoV GI和GII病毒基因组拷贝100 ml（-1）的平均浓度分别为3.56和3.72 log（10）。观察到一个强烈的季节性趋势，并在牡蛎中检测到的NovV GI和GII的浓度与废水中检测到的浓度相关。E.大肠杆菌或FRNA噬菌体。
The concentrations of Escherichia coli, F-specific RNA bacteriophage (FRNA bacteriophage), and norovirus genogroup I (NoV GI) and norovirus genogroup II (NoV GII) in wastewater were monitored weekly over a 1-year period at a wastewater treatment plant (WWTP) providing secondary wastewater treatment. A total of 49 samples of influent wastewater and wastewater that had been treated by primary and secondary wastewater treatment processes (primary and secondary treated wastewater) were analyzed. Using a real-time reverse transcription-quantitative PCR (RT-qPCR), the mean NoV GI and NoV GII concentrations detected in effluent wastewater were 2.53 and 2.63 log(10) virus genome copies 100 ml(-1), respectively. The mean NoV concentrations in wastewater during the winter period (January to March) (n = 12) were 0.82 (NoV GI) and 1.41 (NoV GII) log units greater than the mean concentrations for the rest of the year (n = 37). The mean reductions of NoV GI and GII during treatment were 0.80 and 0.92 log units, respectively, with no significant difference detected in the extent of NoV reductions due to season. No seasonal trend was detected in the concentrations of E. coli or FRNA bacteriophage in wastewater influent and showed mean reductions of 1.49 and 2.13 log units, respectively. Mean concentrations of 3.56 and 3.72 log(10) virus genome copies 100 ml(-1) for NoV GI and GII, respectively, were detected in oysters sampled adjacent to the WWTP discharge. A strong seasonal trend was observed, and the concentrations of NoV GI and GII detected in oysters were correlated with concentrations detected in the wastewater effluent. No seasonal difference was detected in concentrations of E. coli or FRNA bacteriophage detected in oysters.