A molecular timetable for apical bud formation and dormancy induction in poplar
A molecular timetable for apical bud formation and dormancy induction in poplar
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DOI：
10.1105/tpc.107.052811
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发表时间：
2007-08-01
期刊：
影响因子：
11.6
通讯作者：
Rohde, Antje
中科院分区：
文献类型：
--
作者：
Ruttink, Tom;Arend, Matthias;Rohde, Antje
温带多年生植物的生长与休眠期交替，休眠期通常在秋季芽发育期间开始。在揭示杨树（Populus tremula 3 Populus alba）顶芽发育的基本分子程序的系统生物学方法中，组合转录本和代谢物分析被应用于从短日照诱导到完全休眠的高分辨率时间过程。代谢和基因表达动态被用来重建芽发育过程中事件的时间序列。重要的是，芽发育可以分为芽形成、对脱水和寒冷的适应以及休眠。对于每个过程，特定的调控和标记基因和代谢物组都相关联，并为未来的功能研究提供参考框架。光、乙烯和脱落酸信号转导途径通过设置、修改或终止这些过程来连续控制芽的发育。乙烯信号转导暂时位于光信号和脱落酸信号之间，并且可能被短暂的低己糖池激活。通过电子显微镜确定芽内细胞增殖停滞（与休眠相关）和储存化合物积累（与驯化过程相关）的时间和地点。最后，在杨树顶芽、形成层或拟南芥种子的生长到休眠转变期间通常表达的一大组基因的鉴定表明，不同植物器官中潜在的分子机制存在相似之处。
The growth of perennial plants in the temperate zone alternates with periods of dormancy that are typically initiated during bud development in autumn. In a systems biology approach to unravel the underlying molecular program of apical bud development in poplar (Populus tremula 3 Populus alba), combined transcript and metabolite profiling were applied to a high-resolution time course from short-day induction to complete dormancy. Metabolite and gene expression dynamics were used to reconstruct the temporal sequence of events during bud development. Importantly, bud development could be dissected into bud formation, acclimation to dehydration and cold, and dormancy. To each of these processes, specific sets of regulatory and marker genes and metabolites are associated and provide a reference frame for future functional studies. Light, ethylene, and abscisic acid signal transduction pathways consecutively control bud development by setting, modifying, or terminating these processes. Ethylene signal transduction is positioned temporally between light and abscisic acid signals and is putatively activated by transiently low hexose pools. The timing and place of cell proliferation arrest (related to dormancy) and of the accumulation of storage compounds (related to acclimation processes) were established within the bud by electron microscopy. Finally, the identification of a large set of genes commonly expressed during the growth-to-dormancy transitions in poplar apical buds, cambium, or Arabidopsis thaliana seeds suggests parallels in the underlying molecular mechanisms in different plant organs.