Cloacal and buccal swabs are a reliable source of DNA for microsatellite genotyping of reptiles
Cloacal and buccal swabs are a reliable source of DNA for microsatellite genotyping of reptiles
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DOI：
10.1007/s10592-006-9120-2
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发表时间：
2006-12-01
影响因子：
2.2
通讯作者：
Miller, Hilary C.
中科院分区：
文献类型：
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作者：
Miller, Hilary C.
在这项研究中，描述了一种微创方法，用于爬行动物和两栖动物的DNA取样，使用肛肠和口腔拭子。从大鳞鳄（Sphenodon punctatus）的拭子中分离高分子量DNA，在70%乙醇中室温保存约1周。在所有病例中，从肛肠和口腔拭子中成功扩增线粒体和微卫星DNA位点，并且基因型与从血液样本中获得的基因型相匹配。这些结果表明，在对爬行动物进行遗传研究时，肛管和/或口腔拭子是一种有用的替代血液采样和脚趾夹的方法。该方法快速、廉价、易于在现场实施。
In this study, a minimally invasive method for DNA sampling of reptiles and amphibians using cloacal and buccal swabs is described. High molecular weight DNA was isolated from the swabs, which were collected from tuatara (Sphenodon punctatus), and stored in 70% ethanol at room temperature for approximately 1 week. Amplification of mitochondrial and microsatellite DNA loci was successful from both cloacal and buccal swabs, and in all cases the genotypes matched those obtained from blood samples. These results show that cloacal and/or buccal swabbing is a useful alternative to blood sampling and toe clipping for genetic studies on reptiles. This method is rapid, inexpensive and easy to implement in field situations.