Carbendazim Resistance of Fusarium graminearum From Henan Wheat
Carbendazim Resistance of Fusarium graminearum From Henan Wheat
复制标题
河南小麦对禾谷镰刀菌多菌灵的抗性
DOI：
10.1094/pdis-02-19-0391-re
复制
发表时间：
2019-10-01
期刊：
影响因子：
4.5
通讯作者：
Chen, Genqiang
中科院分区：
文献类型：
--
作者：
Liu, Shengming;Fu, Liuyuan;Chen, Genqiang
赤霉病又称赤霉病，是由禾谷镰刀菌引起的小麦重要毁灭性病害之一。1，132株F.测定了2016年、2017年和2018年河南省不同地区田间禾谷孢菌的含量。共31个F。对多菌灵产生抗性的菌株有30个，其中中抗菌株30个，高抗菌株1个。F.禾谷镰刀菌对多菌灵的敏感性为2.7%。1,101株敏感菌株和30株中度抗性菌株的有效浓度（EC_（50））范围分别为0.08 ~ 0.98 μ g·ml ~（-1）和2.73 ~ 13.28 μ g·ml ~（-1）。平均+/- SD EC 50值分别为0.55 +/- 0.13 μ g ml（-1）和5.61 +/- 2.58 μ g ml（-1）。高抗菌株的EC_（50）值为21.12 μ g·ml ~（-1）。通过对31株多菌灵耐药菌株β 2-微管蛋白基因的克隆，分析了多菌灵耐药菌株的点突变类型。在β 2-微管蛋白基因中发现了三种点突变类型，分别位于F167 Y、E198 Q和E198 L。在31株耐药菌株中，β 2-微管蛋白基因F167 Y、E198 Q和E198 L点突变型频率分别为71.0%、25.8%和3.2%。数据表明，F.河南省禾谷镰刀菌对多菌灵产生了抗药性，突变类型以F167 Y单点突变为主。
Fusarium head blight, also called scab, is caused by Fusarium graminearum and is one of the most important destructive diseases of wheat. The frequency of carbendazim resistance in 1,132 isolates of F. graminea rum recovered from fields in different regions of Henan Province in 2016, 2017, and 2018 was determined. A total of 31 F. graminearum isolates resistant to carbendazim were detected, including 30 moderately resistant isolates and one highly resistant isolate. The frequency of resistance of F. graminearum isolates to carbendazim was 2.7%. The range of effective concentration (EC50) values of 1,101 sensitive isolates and 30 moderately resistant isolates was 0.08 to 0.98 mu g ml(-1) and 2.73 to 13.28 mu g ml(-1), respectively. The mean +/- SD EC50 value was 0.55 +/- 0.13 mu g ml(-1) and 5.61 +/- 2.58 g ml(-1), respectively. The EC50 value of the highly resistant isolate was 21.12 mu g ml(-1). Point mutation types of the carbendazim-resistant isolates were characterized by cloning the beta(2)-tubulin gene of 31 resistant isolates. Three point mutation types at amino acids F167Y, E198Q, and E198L in the beta(2)-tubulin gene of resistant isolates were identified. Among 31 resistant isolates, the frequency of point mutation types in F167Y, E198Q, and E198L of the beta(2)-tubulin gene was 71.0, 25.8, and 3.2%, respectively. The data indicate that F. graminearum has developed resistance to carbendazim in Henan Province, and single point mutations at amino acid F167Y were the predominant type of mutation detected.