Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
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DOI：
10.4067/s0716-97602006000500010
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发表时间：
2006-01-01
影响因子：
6.7
通讯作者：
ANDANT, GERARD
中科院分区：
文献类型：
--
作者：
CHARRIER, MAR YVONNE;FONTY, GERARD;ANDANT, GERARD
本文报道了食用蜗牛Cornu aspersum（Syn：H. aspersa）和苹果树（H. pomatia）的16 S rRNA序列分析和表型鉴定。本研究以夏螺和两个种群的钉螺为研究对象。pomatia被认为是。可培养菌主要分布在肠道远端，最高可达5.10（9）CFU g（-1），而瑞典H. pomatia似乎明显较少殖民，这表明其微生物群对气候变化的敏感性较高。除一株外，所有菌株与参考菌株共享>= 97%的序列同一性。它们被排列成两个分类群：γ变形菌门与布丘氏菌属、柠檬酸杆菌属、肠杆菌属、克鲁维氏菌属、肥胖杆菌属、拉乌尔氏菌属和厚壁菌门与肠球菌属、乳球菌属和梭菌属。根据文献，这些属大多被分配到肠道环境或叶际，数据有利于培养蜗牛与土壤和植物接触。没有菌株能够消化滤纸、Avicel纤维素或羧甲基纤维素（CMC）。产乙酸菌和产甲烷古菌没有被培养出来，所以氢的命运仍然是个问题。这种微生物群可能在蜗牛的消化过程（发酵）和能量供应（L-乳酸盐，乙酸盐）中发挥重要作用。蜗牛养殖者选择谷物和植物时，应考虑到肠道微生物群的发酵能力。
The intestinal microbiota of the edible snails Cornu aspersum (Syn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g (-1), but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared >= 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium, Raoultella and the Firmicutes with Enterococcus, Lactococcus, and Clostridium. According to the literature, these genera are mostly assigned to enteric environments or to phyllosphere, data in favour of culturing snails in contact with soil and plants. None of the strains were able to digest filter paper, Avicel cellulose or carboxymethyl cellulose (CMC). Acetogens and methanogenic archaea were not cultivated, so the fate of hydrogen remains questionable. This microbiota could play important roles in the digestive process (fermentation) and the energy supply of the snail (L-lactate, acetate). The choice of cereals and plants by snail farmers should take into account the fermentative abilities of the intestinal microbiota.