Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry
Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essential oil industry
复制标题
DOI：
10.1111/j.1365-2672.2007.03456.x
复制
发表时间：
2007-12-01
影响因子：
4
通讯作者：
Narbad, A.
中科院分区：
文献类型：
--
作者：
Mandalari, G.;Bennett, R. N.;Narbad, A.
目的：研究柑桔加工副产物佛手柑皮中富含黄酮类化合物的部分的抗菌性能，以及酶解糖基化对其对不同细菌和酵母活性的影响。方法与结果：测定佛手柑乙醇组分对革兰氏阴性菌（大肠杆菌、恶臭假单胞菌、肠炎沙门氏菌）、革兰氏阳性菌（无性李斯特菌、枯草芽孢杆菌、金黄色葡萄球菌、乳酸乳球菌）和酿酒酵母的抑菌作用。佛手柑提取物对所有革兰氏阴性菌均有抑菌活性，经酶解糖基化后其抑菌效力增强。新橙皮苷、橙皮苷（苷元）、新橙皮苷、橙皮苷（苷元）、橙皮苷（苷元）、柚皮苷、柚皮苷（苷元）的最低抑菌浓度在200 ~ 800 μ g ml（-1）之间。并对三种佛手柑总黄酮的相互作用进行了评价。结论：Pectinase 62L酶制剂能有效地将佛手柑提取物中常见的苷类转化为其苷元，这种去糖基化反应提高了柑橘类黄酮的抑菌活性。环霉醇、柚皮素和橙皮素的两两组合表现出协同作用和无差异的相互作用，依赖于试验指示生物。研究的意义和影响：佛手柑皮是一种对革兰氏阴性菌有活性的天然抗菌剂的潜在来源。
Aims: To evaluate the antimicrobial properties of flavonoid-rich fractions derived from bergamot peel, a byproduct from the Citrus fruit processing industry and the influence of enzymatic deglycosylation on their activity against different bacteria and yeast.Methods and Results: Bergamot ethanolic fractions were tested against Gram-negative bacteria (Escherichia coli, Pseudomonas putida, Salmonella enterica), Gram-positive bacteria (Listeria innocua, Bacillus subtilis, Staphylococcus aureus, Lactococcus lactis) and the yeast Saccharomyces cerevisiae. Bergamot fractions were found to be active against all the Gram-negative bacteria tested, and their antimicrobial potency increased after enzymatic deglycosylation. The minimum inhibitory concentrations of the fractions and the pure flavonoids, neohesperidin, hesperetin (aglycone), neoeriocitrin, eriodictyol (aglycone), naringin and naringenin (aglycone), were found to be in the range 200 to 800 mu g ml(-1). The interactions between three bergamot flavonoids were also evaluated.Conclusion: The enzyme preparation Pectinase 62L efficiently converted common glycosides into their aglycones from bergamot extracts, and this deglycosylation increased the antimicrobial potency of Citrus flavonoids. Pairwise combinations of eriodictyol, naringenin and hesperetin showed both synergistic and indifferent interactions that were dependent on the test indicator organism.Significance and Impact of the Study: Bergamot peel is a potential source of natural antimicrobials that are active against Gram-negative bacteria.