Isolation, identification and application of novel bacterial consortium TJ-1 for the decolourization of structurally different azo dyes
Isolation, identification and application of novel bacterial consortium TJ-1 for the decolourization of structurally different azo dyes
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DOI：
10.1016/j.biortech.2007.12.074
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发表时间：
2008-10-01
影响因子：
11.4
通讯作者：
Garg, Sanjeev
中科院分区：
文献类型：
--
作者：
Joshi, Taruna;Iyengar, Leela;Garg, Sanjeev
本文报道了一株对酸性橙橙子7（AO 7）及其它偶氮染料具有良好脱色效果的新菌株TJ-1。经16 S rRNA基因序列分析，TJ-1中的3株细菌分别为豚鼠气单胞菌、奇异变形杆菌和球形红球菌。AO 7脱色显着更高的使用财团相比，使用单个菌株，表明这些菌株之间的互补相互作用。在微好氧条件下，在有机碳源存在下，AO 7被观察到脱色。无论是单独的酵母提取物（YE）或YE和葡萄糖的组合相比，单独的葡萄糖，蛋白胨或淀粉导致更高的脱色AO 7。不同初始AO 7浓度的动力学研究表明，即使在200 mg/l浓度下，16 h内也可实现90%以上的脱色。补料分批研究表明，AO 7脱色在第一个循环中需要10 h，在第二个和第三个循环中需要5 h，表明细菌细胞可用于多个循环。该菌对15种偶氮染料以及16种偶氮染料混合物的模拟废水进行了脱色，为TJ-1在工业废水处理中的应用提供了可能。(c)2008爱思唯尔有限公司保留所有权利。
A novel bacterial consortium (TJ-1), which could decolorize Acid Orange 7 (AO7) and manyother azo dyes, was developed. In TJ-1 three bacterial strains were identified as Aeromonas caviae, Proteus mirabilis and Rhodococcus globerulus by 16S rRNA gene sequence analysis. AO7 decolorization was significantly higher with the use of consortium as compared to the use of individual strains, indicating complementary interactions among these strains. AO7 decolorization was observed under microaerophilic condition in the presence of organic carbon source. Either yeast extract (YE) alone or a combination of YE and glucose resulted in much higher decolorization of AO7 as compared to glucose alone, peptone or starch. Kinetic studies with different initial AO7 concentrations showed that more than 90% decolorization could be achieved even at 200 mg/l within 16 h. Fed-batch studies showed that AO7 decolorization required 10 h during the first cycle and 5 h in the second and third cycles, showing that bacterial cells could be used for multiple cycles. The consortium also decolorized fifteen other azo dyes individually as well as a simulated wastewater containing a mixture of all the sixteen azo dyes, thus, conferring the possibility of application of TJ-1 for the treatment of industrial wastewaters. (c) 2008 Elsevier Ltd. All rights reserved.