Association with a sea anemone alters the skin microbiome of clownfish
Association with a sea anemone alters the skin microbiome of clownfish
复制标题
与海葵的关联改变了小丑鱼的皮肤微生物组
作者：
Zoe A. Pratte;N. Patin;Mary E. McWhirt;Alicia M. Caughman;D. Parris;F. Stewart
宿主相关微生物在动物健康中起着重要作用。小丑鱼和海葵形成了一种互惠互利的关系，外部表面经常相互接触。这种互利共生对小丑鱼皮肤粘膜微生物组的影响尚不清楚。我们监测了小丑鱼Amphiprion clarkii在与海葵Entacmaea quadricolor关联（托管）之前，期间和之后的粘膜微生物组。微生物组组成之间的主机和非主机小丑鱼显着不同。在小丑鱼与海葵分离后，后宿主鱼的微生物组收敛到类似于非宿主鱼的微生物组。与非宿主鱼类相比，显着区分宿主与非宿主小丑鱼的细菌序列变体在海葵微生物组和宿主鱼类中更丰富，这表明从海葵转移或富集。这些结果表明，直接接触互利共生的结果显着，但可逆的微生物组的变化，提出了关于潜在的微生物在介导的鱼海葵相互作用的问题。
Host-associated microbes play important roles in animal health. Clownfish and anemones form a mutualistic relationship where external surfaces are in constant contact with one another. The effects of this mutualism on the skin mucosal microbiome of clownfish are unknown. We monitored the mucosal microbiome of the clownfish Amphiprion clarkii before, during and after association (hosting) with the anemone Entacmaea quadricolor. Microbiome composition differed significantly between hosting and non-hosting clownfish. Upon separation of clownfish from anemones, the microbiome of post-hosting fish converged to resemble that of non-hosting fish. Bacterial sequence variants that significantly distinguished hosting from non-hosting clownfish were more abundant in the anemone microbiome and that of hosting fish, compared to non-hosting fish, suggesting transfer from or enrichment by the anemone. These results show that direct contact mutualism results in significant but reversible microbiome shifts, raising questions about a potential microbial role in mediating the fish–anemone interaction.