Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
Brain tubulin and actin cDNA sequences: isolation of recombinant plasmids.
复制标题
脑微管蛋白和肌动蛋白 cDNA 序列：重组质粒的分离。
作者：
I. Ginzburg;A. de Baetselier;M. D. Walker;L. Behar;H. Lehrach;A. Frischauf;U. Littauer
使用富含微管蛋白和肌动蛋白序列的大鼠脑 mRNA 来制备双链 cDNA。通过将 dsDNA 插入 pBR322 质粒的 Pst1 位点并转化大肠杆菌 chi 1776 宿主来构建重组克隆文库。鉴定并表征了携带微管蛋白和肌动蛋白编码序列的克隆。
Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pst1 site of pBR322 plasmid and transformation of E. coli chi 1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.