ACCUMULATION OF A MURINE-MEMBRANE ATTACHMENT SITE FRACTION WHEN CELL-DIVISION IS BLOCKED IN LKYD AND CHA MUTANTS OF SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI
ACCUMULATION OF A MURINE-MEMBRANE ATTACHMENT SITE FRACTION WHEN CELL-DIVISION IS BLOCKED IN LKYD AND CHA MUTANTS OF SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI
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DOI：
10.1128/jb.168.3.1422-1429.1986
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发表时间：
1986-12-01
影响因子：
3.2
通讯作者：
ROTHFIELD, LI
中科院分区：
文献类型：
--
作者：
CHAKRABORTI, AS;ISHIDATE, K;ROTHFIELD, LI
对鼠伤寒沙门氏菌lkyD（Ts）和E. coli cha（Ts）突变体，其似乎在细胞分裂周期的晚期被阻断。在这两种情况下，在非允许的温度下的突变株的生长与积累的特征细胞被膜部分（馏分OML），其中包含内膜，胞壁蛋白，和外膜组件。分离的级分在组成和浮力密度上对应于先前提出的来自野生型菌株的级分（M.H. Bayer，G. P. Costello和M. E. Bayer，J. Bacteriol. 149：758-767，1982：K. Ishidate，E. S.克里格，J.兹里克，S.去B，B。Glauner，T. J.马卡利斯特和L. I. Rothfield，J.Biol.Chem.261：428-443，1986），以包含内膜胞壁蛋白和外膜之间的粘附位点。OML在LkyD-和Cha-细胞中的积累通过阻断DNA合成的处理来防止。干扰DNA合成的影响似乎不涉及SOS反应。
Membrane fractionation studies were performed on Salmonella typhimurium lkyD(Ts) and E. coli cha(Ts) mutants that appeared to be blocked at a late stage of the cell division cycle. In both cases growth of the mutant strains at nonpermissive temperatures was associated with accumulation of a characteristic cell envelope fraction (fraction OML) that contained inner membrane, murein, and outer membrane components. The isolated fraction corresponded in composition and buoyant density to a fraction from wild-type strains that had previously been suggested (M.H. Bayer, G. P. Costello, and M. E. Bayer, J. Bacteriol. 149:758-767, 1982: K. Ishidate, E. S. Creeger, J. Zrike, S. Deb, B. Glauner, T. J. MacAlister, and L. I. Rothfield, J. Biol. Chem. 261: 428-443, 1986) to contain adhesion sites between inner membrane murein, and outer membrane. The accumulation of OML in LkyD- and Cha- cells was prevented by treatments that blocked DNA synthesis. The effects of interference with DNA synthesis did not appear to involve the SOS response.