Yeast m6A Methylated mRNAs Are Enriched on Translating Ribosomes during Meiosis, and under Rapamycin Treatment.
Yeast m6A Methylated mRNAs Are Enriched on Translating Ribosomes during Meiosis, and under Rapamycin Treatment.
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DOI：
10.1371/journal.pone.0132090
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fray RG
中科院分区：
文献类型：
--
作者：
Bodi Z;Bottley A;Archer N;May ST;Fray RG
近年来，对mRNA甲基化的兴趣激增。对40年前发现的突然兴趣部分是由于FTO（脂肪量肥胖）N6-甲基腺苷（m6 A）脱氨酶活性的发现，从而表明肥胖相关疾病与mRNA中m6 A的存在之间的联系。mRNA甲基化研究突然兴起的另一个催化剂是人类、小鼠和酿酒酵母的mRNA甲基化组的释放。然而，这种mRNA“表位标记”的分子功能或功能仍有待发现。有支持性证据表明，m6 A可能是mRNA降解的标志，因为它与YTH结构域蛋白结合，因此与P体伴侣结合。尽管如此，在HeLa细胞中，仅发现甲基化组的亚群与YTHDF 2结合。模式生物酿酒酵母（Saccharomyces cerevisiae）仅具有一个YTH结构域蛋白（Pho 92，Mrb 1），其靶向PHO 4转录物在磷酸盐饥饿下降解。然而，mRNA甲基化仅在减数分裂诱导条件下被发现，并且PHO 4转录物显然未甲基化。在这篇论文中，我们着手调查m6 A是否可以作为S.我们还试图测试它是否可以在非标准孢子形成条件下被诱导。我们发现m6 A的存在和信息的可译性之间存在正相关。我们还发现在长期雷帕霉素处理后的m6 A诱导。
Interest in mRNA methylation has exploded in recent years. The sudden interest in a 40 year old discovery was due in part to the finding of FTO’s (Fat Mass Obesity) N6-methyl-adenosine (m6A) deaminase activity, thus suggesting a link between obesity-associated diseases and the presence of m6A in mRNA. Another catalyst of the sudden rise in mRNA methylation research was the release of mRNA methylomes for human, mouse and Saccharomyces cerevisiae. However, the molecular function, or functions of this mRNA ‘epimark’ remain to be discovered. There is supportive evidence that m6A could be a mark for mRNA degradation due to its binding to YTH domain proteins, and consequently being chaperoned to P bodies. Nonetheless, only a subpopulation of the methylome was found binding to YTHDF2 in HeLa cells.The model organism Saccharomyces cerevisiae, has only one YTH domain protein (Pho92, Mrb1), which targets PHO4 transcripts for degradation under phosphate starvation. However, mRNA methylation is only found under meiosis inducing conditions, and PHO4 transcripts are apparently non-methylated. In this paper we set out to investigate if m6A could function alternatively to being a degradation mark in S. cerevisiae; we also sought to test whether it can be induced under non-standard sporulation conditions. We find a positive association between the presence of m6A and message translatability. We also find m6A induction following prolonged rapamycin treatment.