Development of a fluorescence immunoassay for highly sensitive detection of amantadine using the nanoassembly of carbon dots and MnO2 nanosheets as the signal probe
Development of a fluorescence immunoassay for highly sensitive detection of amantadine using the nanoassembly of carbon dots and MnO2 nanosheets as the signal probe
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DOI：
10.1016/j.snb.2019.01.100
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发表时间：
2019-05-01
影响因子：
8.4
通讯作者：
Shen, Jianzhong
中科院分区：
文献类型：
--
作者：
Dong, Baolei;Li, Hongfang;Shen, Jianzhong
荧光免疫分析法是一种快速、简便、经济的食品化学污染物检测方法。本研究基于碱性磷酸酶（ALP）触发的荧光“开启”信号，建立了一种灵敏检测金刚烷胺（AMD）的竞争荧光ELISA方法。作为荧光底物，碳量子点（CD）被吸附到MnO 2纳米片（NS）表面，形成p-CD @ MnO 2 NS的纳米组装体，导致CD的荧光猝灭。抗体标记的碱性磷酸酶可催化2-磷酸抗坏血酸水解为抗坏血酸。后者可还原和分解MnO 2纳米结构，并伴随着CD从MnO 2纳米结构表面释放出来，导致CD的荧光恢复。荧光强度的变化与溶液中AMD的浓度有关，因此可以应用于基于ALP的ELISA系统中检测AMD。荧光ELISA检测AMD的线性范围为0.048 ng mL（-1）~ 1.1 ng mL（-1），检测限（LOD）为0.035 ng mL（-1）。新的荧光ELISA显示出高灵敏度检测AMD和其他分析物在食品分析的潜力。
Fluorescence immunoassays are rapid, convenient and cost-effective for the sensitive quantitation of chemical contaminants in foodstuff. In this study, a competitive fluorescence ELISA was developed for the sensitive detection of amantadine (AMD) based on the alkaline phosphatase (ALP)-triggered fluorescence "turn-on" signals. As a fluorescence substrate, carbon dots (CDs) were adsorbed onto the surface of the MnO2 nanosheets (NSs) and formed a nanoassembly of p-CDs@MnO2 NSs which results in the fluorescence quench of CDs. The ALP labelled on antibody could catalyze the hydrolysis of the 2-phosphoascorbic acid into ascorbic acid. The latter could then reduce and decompose the MnO2 NSs, which was accompanied by the release of CDs from the surface of MnO2 NSs and led to the fluorescence recovery of CDs. The change of the fluorescence intensity is related to the concentration of AMD in solution and thus could be applied to detect AMD in an ALP-based ELISA system. The fluorescent ELISA showed a linear detection for AMD in the range of 0.048 ng mL(-1) to 1.1 ng mL(-1) with a detection limit (LOD) of 0.035 ng mL(-1). The novel fluorescent ELISA shows potential for the highly sensitive detection of AMD and other analytes in food analysis.