Effects of Intraframe Distortion on Measures of Cone Mosaic Geometry from Adaptive Optics Scanning Light Ophthalmoscopy.
Effects of Intraframe Distortion on Measures of Cone Mosaic Geometry from Adaptive Optics Scanning Light Ophthalmoscopy.
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DOI：
10.1167/tvst.5.1.10
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发表时间：
2016-02
影响因子：
3
通讯作者：
Carroll J
中科院分区：
文献类型：
--
作者：
Cooper RF;Sulai YN;Dubis AM;Chui TY;Rosen RB;Michaelides M;Dubra A;Carroll J
描述由于眼球无意识运动引起的帧内失真对自适应光学扫描光检眼镜（AOSLO）图像的圆锥镶嵌几何测量的影响。我们采集了20名受试者的AOSLO图像序列，固定时间为1.0、2.0和5.0°。专家分级员从每个150帧序列中手动选择10个最小失真的参考帧用于后续配准。使用多个度量在所有配准图像（n = 600）中测量锥体镶嵌几何形状，并使用这些度量的可重复性评估每个参考帧的失真影响。在另外9个受试者中，我们将AOSLO衍生的测量结果与自适应光学（AO）眼底图像（不包含系统施加的帧内失真）的测量结果进行了比较。我们观察到受试者之间在密度重复性（1.2%-8.7%）、细胞间距离（0.8%-4.6%）、六边Voronoi细胞百分比（0.8%-10.6%）和Voronoi细胞面积规则性（VCAR）（1.2%-13.2%）方面存在显著差异。从AOSLO图像中提取的所有指标的平均值（VCAR除外）与从AO眼底图像中提取的指标无显著差异，但个体图像之间存在差异。我们的数据表明，AOSLO图像中发现的帧内失真会影响锥镶嵌指标的准确性和可重复性。使用来自同一视网膜区域的多个图像来近似“无失真”图像是可能的，尽管需要更多的工作来评估这种方法的可行性。即使在具有良好固视的受试者中，来自AOSLO的图像也包含由于扫描期间的眼睛运动而引起的帧内失真。这些伪影的存在强调了在解释来自扫描仪器的结果时需要谨慎。
To characterize the effects of intraframe distortion due to involuntary eye motion on measures of cone mosaic geometry derived from adaptive optics scanning light ophthalmoscope (AOSLO) images. We acquired AOSLO image sequences from 20 subjects at 1.0, 2.0, and 5.0° temporal from fixation. An expert grader manually selected 10 minimally distorted reference frames from each 150-frame sequence for subsequent registration. Cone mosaic geometry was measured in all registered images (n = 600) using multiple metrics, and the repeatability of these metrics was used to assess the impact of the distortions from each reference frame. In nine additional subjects, we compared AOSLO-derived measurements to those from adaptive optics (AO)-fundus images, which do not contain system-imposed intraframe distortions. We observed substantial variation across subjects in the repeatability of density (1.2%–8.7%), inter-cell distance (0.8%–4.6%), percentage of six-sided Voronoi cells (0.8%–10.6%), and Voronoi cell area regularity (VCAR) (1.2%–13.2%). The average of all metrics extracted from AOSLO images (with the exception of VCAR) was not significantly different than those derived from AO-fundus images, though there was variability between individual images. Our data demonstrate that the intraframe distortion found in AOSLO images can affect the accuracy and repeatability of cone mosaic metrics. It may be possible to use multiple images from the same retinal area to approximate a “distortionless” image, though more work is needed to evaluate the feasibility of this approach. Even in subjects with good fixation, images from AOSLOs contain intraframe distortions due to eye motion during scanning. The existence of these artifacts emphasizes the need for caution when interpreting results derived from scanning instruments.