RED-CELL NADP+ AND NADPH IN GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENCY
RED-CELL NADP+ AND NADPH IN GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENCY
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DOI：
10.1172/jci107998
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发表时间：
1975-01-01
影响因子：
15.9
通讯作者：
MARENI, C
中科院分区：
文献类型：
--
作者：
KIRKMAN, HN;GAETANI, GD;MARENI, C
Extraction in the presence of sodium hydroxide and cysteine allows estimates of NADPH and total NADP in human red cells without the erroneously high values of NADP+ obtained with earlier methods. An application of this technique to G6PD-deficient cells reveals that most of the nucleotide is in the oxidized form. In contrast, normal red cells have nearly all of the nucleotide in the reudced form. In addition to providing information concerning the intracellular regulation of the hexose monophosphate shunt, these findings support the concept that G6PD deficiency is a product-deficiency disorder.
