GENETIC ARCHITECTURE OF METABOLIC RATE: ENVIRONMENT SPECIFIC EPISTASIS BETWEEN MITOCHONDRIAL AND NUCLEAR GENES IN AN INSECT
GENETIC ARCHITECTURE OF METABOLIC RATE: ENVIRONMENT SPECIFIC EPISTASIS BETWEEN MITOCHONDRIAL AND NUCLEAR GENES IN AN INSECT
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DOI：
10.1111/j.1558-5646.2010.01135.x
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发表时间：
2010-12-01
期刊：
影响因子：
3.3
通讯作者：
Hosken, David J.
中科院分区：
文献类型：
--
作者：
Arnqvist, Goran;Dowling, Damian K.;Hosken, David J.
线粒体DNA（mtDNA）变异参与适应性进化变化的程度目前正在重新评估。特别是，新出现的证据表明，线粒体DNA基因与核基因共同进化，它们与核基因相互作用，在线粒体中形成产生能量的酶复合物。这表明线粒体和核基因之间的基因组间上位性可能会影响整个生物体的代谢表型。在这里，我们使用交叉组合的线粒体和核谱系的种子甲虫Callosobruchus maculatus和测定代谢率在两个不同的温度制度。代谢率的线粒体和核谱系和温度制度之间的相互作用的影响。序列数据表明，线粒体遗传变异在决定这种相互作用的结果中起作用。我们对代谢率的遗传解剖揭示了高度的复杂性，包括两个基因组之间的遗传相互作用，以及基因型x基因型x环境相互作用。这些结果的进化含义是双重的。首先，由于代谢率是生命史的根源，我们的研究结果提供了对生命史进化复杂性的见解，特别是热适应。其次，我们的研究结果表明，可能有助于维持非中性mtDNA多态性的机制。
The extent to which mitochondrial DNA (mtDNA) variation is involved in adaptive evolutionary change is currently being reevaluated. In particular, emerging evidence suggests that mtDNA genes coevolve with the nuclear genes with which they interact to form the energy producing enzyme complexes in the mitochondria. This suggests that intergenomic epistasis between mitochondrial and nuclear genes may affect whole-organism metabolic phenotypes. Here, we use crossed combinations of mitochondrial and nuclear lineages of the seed beetle Callosobruchus maculatus and assay metabolic rate under two different temperature regimes. Metabolic rate was affected by an interaction between the mitochondrial and nuclear lineages and the temperature regime. Sequence data suggests that mitochondrial genetic variation has a role in determining the outcome of this interaction. Our genetic dissection of metabolic rate reveals a high level of complexity, encompassing genetic interactions over two genomes, and genotype x genotype x environment interactions. The evolutionary implications of these results are twofold. First, because metabolic rate is at the root of life histories, our results provide insights into the complexity of life-history evolution in general, and thermal adaptation in particular. Second, our results suggest a mechanism that could contribute to the maintenance of nonneutral mtDNA polymorphism.