The role of protein kinase Cα in U-87 glioma invasion
The role of protein kinase Cα in U-87 glioma invasion
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DOI：
10.1016/s0736-5748(99)00054-4
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发表时间：
1999-08-01
影响因子：
1.8
通讯作者：
Rosenblum, ML
中科院分区：
文献类型：
--
作者：
Cho, KK;Mikkelsen, T;Rosenblum, ML
为探讨蛋白激酶Cα(PKCα)在功能性胶质瘤细胞侵袭中的作用，建立了稳定的PKCα正、反义转基因U-87细胞系，并用Western印迹和活性检测来表征PKCα的表达。入侵分析包括屏障迁移(Koochekour er ai.细胞外基质蛋白抑制人脑胶质瘤细胞系的增殖、上调迁移并诱导其形态改变。欧元。J.Cancel，1995，31,375-380；Merzak et NL.，CD44在体外介导人脑胶质瘤细胞的黏附和侵袭。癌症研究，1994，54,3988-3992；Merzak等人，细胞表面神经节苷脂在体外参与控制人脑胶质瘤细胞的侵袭。神经科。Let.，1994,177，11-16)和球体对抗法研究PKCα表达与肿瘤侵袭性的关系。与对照组相比，PKCα高表达克隆的屏障迁移率增加(1.5倍)。PKCα抑制的克隆表现出较低的侵袭性
To investigate the hypothesis that protein kinase C alpha (PKC alpha) is functional glial tumor cell invasion, stable PKC alpha sense and antisense transfected U-87 cell lines were established and PKC alpha expression characterized by Western blot and PKC activity assays. Invasion assays including barrier migration (Koochekpour er ai.. Extracellular matrix proteins inhibit proliferation, upregulate migration and induce morphological changes in human glioma lines. Eur. J. Cancel,1995, 31, 375-380; Merzak et nl., CD44 mediates human glioma cell adhesion and invasion in vitro. Cancer Res., 1994, 54, 3988-3992; Merzak et al., Cell surface gangliosides are involved in the control of human glioma cell invasion in vitro. Neurosci. Lett., 1994, 177, 11-16), and spheroid confrontation were used to study the relationship between PKC alpha expression and invasiveness. PKC alpha overexpressing clones show increased barrier migration (1.5x) relative to the control transfected clones. PKC alpha inhibited clones exhibited reduced invasiveness, to