Intestinal and Peripheral Immune Response to MON810 Maize Ingestion in Weaning and Old Mice
Intestinal and Peripheral Immune Response to MON810 Maize Ingestion in Weaning and Old Mice
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DOI：
10.1021/jf802059w
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发表时间：
2008-12-10
影响因子：
6.1
通讯作者：
Mengheri, Elena
中科院分区：
文献类型：
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作者：
Finamore, Alberto;Roselli, Marianna;Mengheri, Elena
本研究评估了脆弱条件下小鼠对转基因玉米的肠道和外周免疫反应。断奶小鼠和老龄小鼠分别饲喂含有MON810或其亲代对照玉米的饲粮或含有无转基因玉米的颗粒饲粮30天和90天。对照玉米喂养小鼠的肠上皮内、脾脏和血液淋巴细胞的免疫表型与颗粒喂养小鼠相似。与对照玉米相比，MON810玉米分别诱导断奶小鼠和老龄小鼠肠道和外周部位T细胞和B细胞百分比以及CD4（+）、CD8（+）、γ δ T和α β T亚群百分比的变化。饲喂MON810后血清IL-6、IL-13、IL-12p70和MIP-1 β均升高。这些结果表明，肠道和外周免疫反应对转基因作物的摄入以及消费者的年龄在转基因生物安全性评估中的重要性。
This study evaluated the gut and peripheral immune response to genetically modified (GM) maize in mice in vulnerable conditions. Weaning and old mice were fed a diet containing MON810 or its parental control maize or a pellet diet containing a GM-free maize for 30 and 90 days. The immunophenotype of intestinal intraepithelial, spleen, and blood lymphocytes of control maize fed mice was similar to that of pellet fed mice. As compared to control maize, MON810 maize induced alterations in the percentage of T and B cells and of CD4(+), CD8(+), gamma delta T, and alpha beta T subpopulations of weaning and old mice fed for 30 or 90 days, respectively, at the gut and peripheral sites. An increase of serum IL-6, IL-13, IL-12p70, and MIP-1 beta after MON810 feeding was also found. These results suggest the importance of the gut and peripheral immune response to GM crop ingestion as well as the age of the consumer in the GMO safety evaluation.