Preliminary study for evaluation of avian influenza virus inactivation in contaminated poultry products using electron beam irradiation
Preliminary study for evaluation of avian influenza virus inactivation in contaminated poultry products using electron beam irradiation
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DOI：
10.1080/03079450902912150
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发表时间：
2009-01-01
期刊：
影响因子：
2.8
通讯作者：
Reddy, S. M.
中科院分区：
文献类型：
--
作者：
Brahmakshatriya, V.;Lupiani, B.;Reddy, S. M.
禽流感的传播与受感染家禽和家禽产品的流动有关，因此实行了严格的国际贸易限制。此外，通过食用或处理受感染的家禽而将禽流感病毒（AIV）毒株传播给哺乳动物的可能性虽然很少发生，但仍是一个重要的贸易问题。传统的禽类产品净化方法，如热加工，对灭活禽流感病毒是有效的。然而，电子束（电子束）辐照等替代技术在保持新鲜家禽产品的感官特性方面具有一些优势。本研究旨在评价高能（10兆电子伏特）电子束辐照在禽肉和蛋制品中灭活AIV和降低病毒载量的适用性。用低致病性A/chicken/TX/2002 H5N3 AIV加入市售蛋清和火鸡肉样品，并暴露于不同剂量的高能电子束照射下，照射量从0 kGy到8 kGy不等。辐照样品中的病毒滴度呈线性剂量依赖性降低。使活AIV负荷减少90%（即d10值）所需的剂量在磷酸盐缓冲液中为2.3千吉吉，在蛋清中为1.6千吉吉，在火鸡肉碎样品中为2.6千吉吉。电子束辐照灭活AIV的效果与先前报道的电子束灭活脊髓灰质炎病毒和轮状病毒的效果相似。这些结果说明高能（10兆电子伏特）电子束辐照作为家禽产品去污技术灭活AIV的潜在适用性。
Spread of avian influenza is associated with movement of infected poultry and poultry products, and, as a result, strict international trade restrictions are in place. In addition, the possibility of transmission of avian influenza virus (AIV) strains to mammalian species by the consumption or handling of infected poultry, although a rare occurrence, is an important trade concern. Traditional methods for poultry product decontamination, such as thermal processing, are effective in inactivating AIV. However, alternative technologies such as electron beam (E-beam) irradiation offer some advantages in maintaining organoleptic properties of fresh poultry products. This study was designed to evaluate the applicability of high-energy (10 MeV) E-beam irradiation to inactivate AIV and reduce virus loads in egg products and poultry meat. Commercially available egg-white and ground turkey meat samples were spiked with the low-pathogenic A/chicken/TX/2002 H5N3 AIV and exposed to varying doses of high-energy E-beam irradiation in increments ranging from 0 kGy to 8 kGy. The viral titres in irradiated samples showed a linear dose-dependent reduction. The dose required to achieve 90% reduction (i.e. the D 10 value) of viable AIV loads was 2.3 kGy in phosphate buffer, 1.6 kGy in egg-white and 2.6 kGy in ground turkey meat samples. The effectiveness of E-beam irradiation to inactivate AIV was similar to the previously reported effectiveness of the E-beam against poliovirus and rotavirus. These results illustrate the potential applicability of high-energy (10 MeV) E-beam irradiation as a poultry product decontamination technology to inactivate AIV.