Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens
Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens
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DOI：
10.1095/biolreprod66.6.1716
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发表时间：
2002-06-01
影响因子：
3.6
通讯作者：
Breton, S
中科院分区：
文献类型：
--
作者：
Pastor-Soler, L;Isnard-Bagnis, C;Breton, S
男性流出道上皮细胞对液体和溶质的重吸收对精子的成熟、浓缩和储存至关重要。水通道有助于水运动穿过许多组织中的上皮。水通道蛋白9（AQP 9）在附睾主细胞的顶膜以及男性生殖道的重吸收和分泌上皮细胞中大量表达。在这项研究中，我们发现，通过渗透压微型泵给予成年大鼠50 mg/kg/d的非甾体抗雄激素氟替卡松，持续2周，可显著降低附睾中AQP 9的含量。这种下调通过低温组织切片的免疫荧光和附睾刷状缘膜制备的Western印迹法观察到。此外，去势成年大鼠显示较低水平的附睾AQP 9与成年对照组相比，而全身睾酮治疗的去势成年大鼠诱导的AQP 9的表达恢复到对照水平。这些数据表明，AQP 9的表达，一个可能的候选人顶端transepithelial流体和溶质运输在几个地区的男性生殖道，调节雄激素在成年大鼠附睾。
Reabsorption of fluid and solutes across the epithelium lining the male excurrent duct is important for adequate sperm maturation, concentration, and storage. Water channels contribute to water movement across epithelia in many tissues. Aquaporin 9 (AQP9) is abundantly expressed in the apical membrane of principal cells that line the epididymis, and in reabsorptive and secretory epithelial cells of the male reproductive tract. In this study we show that the nonsteroidal antiandrogen flutamide, given to adult rats at a dose of 50 mg kg(-1) day(-1) for 2 wk via osmotic minipumps significantly decreased the amount of AQP9 in the epididymis. This down-regulation was observed by immunofluorescence of cryostat tissue sections and by Western blotting of epididymal brush border membrane preparations. In addition, castrated adult rats showed lower levels of epididymal AQP9 compared with adult controls, whereas systemic testosterone treatment of castrated adult rats induced a recovery of the expression of AQP9 to control levels. These data indicate that the expression of AQP9, a likely candidate for apical transepithelial fluid and solute transport in several regions of the male reproductive tract, is modulated by androgens in the adult rat epididymis.