Total internal reflection fluorescence: applications in cellular biophysics.
Total internal reflection fluorescence: applications in cellular biophysics.
复制标题
全内反射荧光：在细胞生物物理学中的应用。
DOI：
10.1016/s0958-1669(97)80158-9
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发表时间：
1997
影响因子：
7.7
通讯作者：
Lagerholm,BC
中科院分区：
文献类型：
--
作者：
Thompson,NL;Lagerholm,BC
发生在细胞膜表面或靠近细胞膜表面的分子相互作用有望具有不同于在本体溶液中发生的性质。在分子水平上研究表面相关过程的一种特别有用的技术是全内反射荧光。在这种方法中，来自内反射激发源的逝去场选择性地激发表面上或表面附近的荧光分子。近年来，随着荧光显微镜和光谱学的各种技术的使用，人们开始使用逝去的激发来探测自然或模拟生物表面上生化反应的基本物理化学性质。这些研究提供了对细胞功能的更好理解。其他领域在全内反射荧光方法学方面的几个最新进展可能会在细胞生物物理学中找到未来的应用。
Molecular interactions occurring on or near cell membrane surfaces are expected to have different properties from those occurring in bulk solutions. One particularly useful technique for studying surface-associated processes at the molecular level is total internal reflection fluorescence. In this method, the evanescent field from an internally reflected excitation source selectively excites fluorescent molecules on or near a surface. Evanescent excitation has been used recently with a variety of techniques in fluorescence microscopy and spectroscopy to probe the fundamental physicochemical properties of biochemical reactions at natural or model biological surfaces. These studies are providing enhanced understanding of cellular function. Several recent developments in total internal reflection fluorescence methodology from other fields are likely to find future application in cellular biophysics.