Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers
Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers
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DOI：
10.1046/j.1365-294x.1998.00415.x
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发表时间：
1998-08-01
影响因子：
4.9
通讯作者：
Taberlet, P
中科院分区：
文献类型：
--
作者：
Escaravage, N;Questiau, S;Taberlet, P
在欧洲阿尔卑斯山脉，罗鹃花（Rhonodendron ferrugineum）可以构成密集的种群，几乎100%的覆盖率。分层的发育模式和由此产生的种群结构的复杂性使得即使通过挖掘也难以识别不同的无性系。因此，从AFLP标记推断了法国阿尔卑斯地区一个铁秧居群的基因型结构。在第一步中，我们使用由一个选择性引物对生成的AFLP图谱分析了400个样品。25个条带中有17个是多态性的（68%）。我们共鉴定出32种多位点基因型。在第二步中，通过将两个额外的引物对应用于每个基因型的两个最远的样本来验证32个基因型。克隆对间的平均相似度（共用带比例）为0.85（范围为0.52 ~ 0.94）。无性系的空间分布表明，无性扩展主要发生在坡下。根据年平均生长2.6 cm/年和最宽无性系的大小，我们估计最老个体的年龄至少为300岁。一个基因型可以占据一个大的表面，有时形成密集的斑块，这表明该物种采用密集的生长形式，一些基因的混合有限。
In the European Alps, Rhonodendron ferrugineum can constitute dense populations with almost 100% of cover. The developmental pattern by layering and the resulting complexity of population structure make it difficult to identify distinct clones even by excavation. Therefore genotypic structure of a R. ferrugineum population, in the French Alps, was inferred from AFLP markers. In a first step, we analysed 400 samples using AFLP profiles generated by one selective primer pair. Seventeen bands out of 25 were polymorphic (68%). We identified a total of 32 multilocus genotypes. In a second step, the 32 genotypes were verified by applying two additional primer pairs to the two most distant samples from each genotype. The mean similarity (proportion of band sharing) between pairs of clones was 0.85 (range from 0.52 to 0.94). The spatial distribution of clones showed that vegetative spreading mainly occurred down a slope. Based on an annual shoot mean growth of 2.6 cm/year and the size of the widest clone, we estimated the age of the oldest individual to be at least 300 years. A single genotype can occupy a large surface and sometimes form a dense patch, suggesting that this species adopts a phalanx growth form with limited intermingling of some genets.