Growth velocities of branched actin networks
Growth velocities of branched actin networks
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DOI：
10.1016/s0006-3495(03)70018-6
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发表时间：
2003-05-01
影响因子：
3.4
通讯作者：
Carlsson, AE
中科院分区：
文献类型：
--
作者：
Carlsson, AE
肌动蛋白网络的增长对障碍物，刺激局部分支研究使用几种变体的动力学速率模型的基础上的取向依赖的数密度的细丝。该模型强调了自由丝端密度的分支和盖帽的影响。这些变体在侧支与端支和维度的处理上有所不同，并假设新的分支是由现有分支（自催化行为）或独立于现有分支（成核行为）产生的。在自催化模型中，网络的增长速度与障碍物施加的反作用力严格无关，网络密度与力成正比。的增长速度上的支化率和封盖率的依赖性进行评估的速率方程的数值解。在侧分支模型中，生长速度随着分支率的减小而逐渐下降到零，而在端分支模型中，下降是突然的。当帽化率趋于零时，发现速度的行为对分支区的厚度敏感。实验提出使用这些结果来阐明的分支过程的性质。
The growth of an actin network against an obstacle that stimulates branching locally is studied using several variants of a kinetic rate model based on the orientation-dependent number density of filaments. The model emphasizes the effects of branching and capping on the density of free filament ends. The variants differ in their treatment of side versus end branching and dimensionality, and assume that new branches are generated by existing branches (autocatalytic behavior) or independently of existing branches (nucleation behavior). In autocatalytic models, the network growth velocity is rigorously independent of the opposing force exerted by the obstacle, and the network density is proportional to the force. The dependence of the growth velocity on the branching and capping rates is evaluated by a numerical solution of the rate equations. In side-branching models, the growth velocity drops gradually to zero with decreasing branching rate, while in end-branching models the drop is abrupt. As the capping rate goes to zero, it is found that the behavior of the velocity is sensitive to the thickness of the branching region. Experiments are proposed for using these results to shed light on the nature of the branching process.