ENDOTHELIAL-CELL MEMBRANES CONTAIN PODOCALYXIN - THE MAJOR SIALOPROTEIN OF VISCERAL GLOMERULAR EPITHELIAL-CELLS
ENDOTHELIAL-CELL MEMBRANES CONTAIN PODOCALYXIN - THE MAJOR SIALOPROTEIN OF VISCERAL GLOMERULAR EPITHELIAL-CELLS
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DOI：
10.1083/jcb.102.2.484
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发表时间：
1986-02-01
影响因子：
7.8
通讯作者：
KERJASCHKI, D
中科院分区：
文献类型：
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作者：
HORVAT, R;HOVORKA, A;KERJASCHKI, D
足糖萼蛋白是肾小球足细胞糖萼中的主要唾液蛋白。在这里，我们报告其肾小球外的本地化，使用单特异性抗体，这是通过亲和纯化的IgG对肾小球足糖萼蛋白的硝酸纤维素转移。通过间接免疫荧光，足糖萼蛋白被发现在几个器官的血管（肺，心脏，肾，小肠，脑，胰腺，主动脉，肝门静脉周围血管，和脾滤泡的中央动脉，但不是在内皮细胞，后者的器官的窦）。通过免疫电子显微镜，在扩散（“包埋前”）和表面（“包埋后”）程序中使用免疫金缀合物，足糖萼蛋白定位于内皮细胞的管腔膜域，呈斑片状分布。足糖萼蛋白的存在通过免疫印迹法在肺组织的SDS提取物中确认。我们的结论是：（a）足糖萼蛋白是内皮细胞质膜的一个广泛的组成部分，（B）它被限制在管腔膜域，和（c）它是不均匀地分布在内皮细胞表面。
Podocalyxin is the major sialoprotein in the glycocalyx of glomerular podocytes. Here we report on its extraglomerular localization, using a monospecific antibody which was obtained by affinity purification of IgG on nitrocellulose transfers of glomerular podocalyxin. By indirect immunofluorescence, podocalyxin was found in the blood vessels of several organs (lung, heart, kidney, small intestine, brain, pancreas, aorta, the periportal blood vessels in liver, and the central arteries of follicles of the spleen, but not in the endothelia that line the sinusoids of the latter organs). By immunoelectron microscopy.sbd.using immunogold conjugates in diffusion ("pre-embedding") and surface ("postembedding") procedures.sbd.podocalyxin was localized on the luminal membrane domain of endothelial cells, in a patchy distribution. The presence of podocalyxin was confirmed in SDS extracts of lung tissues by immunoblotting. We conclude that (a) podocalyxin is a widespread component of endothelial plasma membranes, (b) it is restricted to the luminal membrane domain, and (c) it is distributed unevenly on the endothelial cell surface.