CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR BOVINE ADRENAL PRE-PROENKEPHALIN
CLONING AND SEQUENCE-ANALYSIS OF CDNA FOR BOVINE ADRENAL PRE-PROENKEPHALIN
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DOI：
10.1038/295202a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
NUMA, S
中科院分区：
文献类型：
--
作者：
NODA, M;FURUTANI, Y;NUMA, S
克隆的牛肾上腺髓质前脑啡肽原基因的核苷酸序列表明，前体蛋白含有4个拷贝的甲硫氨酸脑啡肽和1个拷贝的亮氨酸脑啡肽、甲硫氨酸脑啡肽-Arg6-Phe7和甲硫氨酸脑啡肽-Arg6-Gly7-Leu8，这是一种以前未发现的阿片肽。脑啡肽和延伸的脑啡肽序列均由成对的碱性氨基酸残基结合。前脑啡肽原可能代表一种多激素前体，如促肾上腺皮质激素-β-促脂激素前体。
The nucleotide sequence of cloned cDNA for preproenkephalin from bovine adrenal medulla indicates that the precursor protein contains four copies of Met-enkephalin and one copy each of Leu-enkephalin, Met-enkephalin-Arg6-Phe7and Met-enkephalin-Arg6-Gly7-Leu8, a previously undetected opioid peptide. The enkephalin and extended enkephalin sequences are each bounded by paired basic amino acid residues. Preproenkephalin may represent a multi-hormone precursor, like the corticotropin-β-lipotropin precursor.