The transcriptional response to salicylic acid plays a role in Fusarium yellows resistance in Brassica rapa L.
The transcriptional response to salicylic acid plays a role in Fusarium yellows resistance in Brassica rapa L.
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DOI：
10.1007/s00299-020-02658-1
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发表时间：
2021-01-18
影响因子：
6.2
通讯作者：
Fujimoto, Ryo
中科院分区：
文献类型：
--
作者：
Miyaji, Naomi;Shimizu, Motoki;Fujimoto, Ryo
由尖镰孢 f. 引起的镰刀菌黄化。 sp。球菌凝集素（Foc）是一种重要的疾病。先前的研究表明，在白菜镰刀菌黄化抗性品系中，Foc感染后与水杨酸（SA）反应相关的基因比易感品系更容易被诱导。然而，SA 诱导的基因尚未在全基因组水平上得到鉴定，并且尚不清楚它们是否因 Foc 接种而上调。对白菜镰刀菌黄化敏感品系“Misugi”和抗性品系“Nanane”进行有和无SA处理的转录组分析，以深入了解SA敏感性/反应与镰刀菌黄化抗性之间的关系。 'Nanane 的上调基因与 SA 反应相关，下调基因与茉莉酸 (JA) 或乙烯 (ET) 反应相关，但 'Misugi' 中的差异表达基因则不然。该结果表明镰刀菌黄化抗性和易感品系具有不同的SA反应，并且仅在镰刀菌黄化抗性品系中发现SA和JA/ET反应之间的拮抗转录。 SA 反应基因通过 Foc 接种在镰刀菌黄化抗性 (RJKB-T23) 和敏感品系 (RJKB-T24) 中诱导。相比之下，RJKB-T23 特异性的 39 个 SA 诱导基因可能在针对 Foc 的防御反应中发挥作用。在这项研究中，在全基因组水平上鉴定了 SA 诱导的基因，并提出了在抗性品系中观察到的对 Foc 的防御反应可能是由 SA 诱导的基因介导的可能性。这些结果将有助于未来关于 SA 在 Foc 中的重要性或白菜中其他疾病抗性的研究。
Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease. Previous studies showed that genes related to salicylic acid (SA) response were more highly induced following Foc infection in Brassica rapa Fusarium yellows resistant lines than susceptible lines. However, SA-induced genes have not been identified at the whole genome level and it was unclear whether they were up-regulated by Foc inoculation. Transcriptome analysis with and without SA treatment in the B. rapa Fusarium yellows susceptible line 'Misugi' and the resistant line 'Nanane' was performed to obtain insights into the relationship between SA sensitivity/response and Fusarium yellows resistance. 'Nanane's up-regulated genes were related to SA response and down-regulated genes were related to jasmonic acid (JA) or ethylene (ET) response, but differentially expressed genes in 'Misugi' were not. This result suggests that Fusarium yellows resistant and susceptible lines have a different SA response and that an antagonistic transcription between SA and JA/ET responses was found only in a Fusarium yellows resistant line. SA-responsive genes were induced by Foc inoculation in Fusarium yellows resistant (RJKB-T23) and susceptible lines (RJKB-T24). By contrast, 39 SA-induced genes specific to RJKB-T23 might function in the defense response to Foc. In this study, SA-induced genes were identified at the whole genome level, and the possibility, the defense response to Foc observed in a resistant line could be mediated by SA-induced genes, is suggested. These results will be useful for future research concerning the SA importance in Foc or other diseases resistance in B. rapa.