Effects of hydroxyapatite implants on primary mineralization during rat tibial healing: biochemical and morphometric analyses.
Effects of hydroxyapatite implants on primary mineralization during rat tibial healing: biochemical and morphometric analyses.
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羟基磷灰石植入物对大鼠胫骨愈合过程中初级矿化的影响：生化和形态分析。
DOI：
10.1002/jbm.820270808
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Boyan,B
中科院分区：
文献类型：
--
作者：
Schwartz,Z;Braun,G;Kohavi,D;Brooks,B;Amir,D;Sela,J;Boyan,B
40 - 60目羟基磷灰石（HA）颗粒（Calcitek，Inc.，卡尔斯巴德，CA）对骨愈合过程中初级矿化过程的影响。通过监测基质囊泡（与初始钙化相关的细胞外细胞器）的形态和生化变化来评估对HA的反应。植入HA后，在第3、6、14和21天，从植入物附近的组织和对侧肢体的骨内膜以及非植入对照组（t = 0）中分离基质囊泡富集膜（MVEM）。MVEM碱性磷酸酶和磷脂酶A2特异性活性在第6天（峰值）和第14天增加;磷脂酰丝氨酸含量也在第6天和第14天（峰值）升高。在对侧肢体中观察到类似的变化，但幅度较小。同时观察形态学变化。基质囊泡/μ m2基质的数量在第6天（峰值）和第14天增加。基质囊泡的平均直径在第6天（峰值）、第14天和第21天升高。钙化前沿的平均距离在第6天增加，但在第14天和第21天减少。这些结果表明，HA的行为与骨结合植入物相似，因为它会刺激基质囊泡酶、增加磷脂酰丝氨酸含量并增加基质囊泡的数量。然而，增加仅发生在植入后6天，表明与正常愈合相比，反应延迟。这种延迟通过形态测量证实。HA导致与骨髓消融相关的反应降低。此外，HA的局部和全身效应相当，但强度不同。这些观察结果表明，成骨细胞能够补偿HA的抑制作用，并且原发性钙化涉及正常的基质囊泡产生和成熟，如果稍微延迟和幅度降低的话。支持原生矿物质形成的能力可能有助于HA与周围骨组织的成功结合。John Wiley & Sons，Inc.
The effect of 40‐ to 60‐mesh hydroxyapatite (HA) granules (Calcitek, Inc., Carlsbad, CA) on the process of primary mineralization during bone healing was examined following insertion of the HA granules into rat tibial bone after marrow ablation. Response to HA was assessed by monitoring morphometric and biochemical changes in matrix vesicles, which are extracellular organelles associated with initial calcification. Following insertion of HA, matrix vesicle‐enriched membranes (MVEMs) were isolated from the tissue adjacent to the implant and from the endosteum of the contralateral limb at 3,6,14, and 21 days and from a nonimplanted control group (t = 0). MVEM alkaline phosphatase‐ and phospholipase A2‐specific activities were increased on days 6 (peak) and 14; phosphatidylserine content was also elevated on days 6 and 14 (peak). Comparable changes were seen in the contralateral limb but at lesser magnitudes. Morphological changes were observed as well. The number of matrix vesicles/μm2matrix increased on days 6 (peak) and 14. The mean diameter of the matrix vesicles was elevated on days 6 (peak), 14, and 21. Mean distance from the calcifying front increased on day 6 but was decreased on days 14 and 21. These results indicated that HA behaves like bone‐bonding implants in that there is a stimulation of matrix vesicle enzymes, increased phosphatidylserine content, and increase numbers of matrix vesicles. However, the increases occur only after 6 days postimplantation, indicating a delay in response when compared to normal healing. This delay is confirmed by the morphometric measurements. HA causes a reduction in the response associated with marrow ablation. In addition, the effects of HA are comparable locally and systemically but with different intensity. These observations suggest that osteogenic cells are able to compensate for the inhibitory effects of HA and primary calcification involves normal matrix vesicle production and maturation, if somewhat delayed and reduced in magnitude. The ability to support primary mineral formation may contribute to the successful bonding of HA with surrounding osseous tissue. © 1993 John Wiley & Sons, Inc.